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FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), Super Bright™ 600, eBioscience™
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FIGURE: 1 / 2
FceR1 alpha Antibody (63-5899-42) in Flow
Normal human peripheral blood cells were stained with IgE Monoclonal Antibody, APC (Product # 17-6986-42) and Mouse IgG2b kappa Isotype Control, Super Bright 600 (Product # 63-4732-82) (left) or FceR1 alpha Monoclonal Antibody, Super Bright 600 (right). Cells in the lymphocyte gate were used for analysis (note: basophils reside in FSC/SSC position of lymphocytes).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
63-5899-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
AER-37 (CRA1)
Conjugate
Super Bright™ 600
Super Bright™ 600
Super Bright™ 600
Excitation/Emission Max
414/601 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The AER-37 monoclonal antibody reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI apha is expressed on mast and basophil cells and is upregulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions.
Applications Reported: This AER-37 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This AER-37 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Fc epsilon RI alpha, also known as FceR1 alpha, is a subunit of the high-affinity receptor for IgE, primarily expressed on mast cells and basophils. Its expression is upregulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits, which are essential for signal transduction. The beta and gamma subunits contain immunoreceptor tyrosine-based activation motifs (ITAMs). The Fc epsilon RI complex plays a crucial role in triggering IgE-mediated allergic reactions. When allergen-bound IgE molecules bring together two or more high-affinity IgE receptors, it leads to the release of mediators such as histamine, which are responsible for allergy symptoms. This process couples allergens and mast cells to initiate inflammatory responses characteristic of allergic disorders like hay fever and asthma. The release of histamine and proteases also leads to the synthesis of prostaglandins and leukotrienes, which are potent effectors of the hypersensitivity response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: fc epsilon r1; Fc epsilon RI alpha-chain; Fc fragment of IgE receptor Ia; Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide; Fc IgE receptor, alpha polypeptide; Fc Receptor Epsilon I alpha; Fc-epsilon RI-alpha; FcepsilonRI alpha chain; FcepsilonRIalpha; FceR1a; FceRI; high affinity immunoglobulin epsilon receptor alpha-subunit; igE Fc receptor subunit alpha; immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide; mast cell IgE receptor alpha-chain; RP11-550P17.3; unnamed protein product
Gene Aliases: FCE1A; FcERI; FCERIA
UniProt ID: (Human) P12319
Entrez Gene ID: (Human) 2205
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