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          • Primary Antibodies ›
          • RhoA Antibodies

          Proteintech

          RHOA Monoclonal Antibody (1B3D7)

          10 References
          View all (37) RhoA antibodies

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          Cite RHOA Monoclonal Antibody (1B3D7)

          Additional Information:
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          • Antibody Testing Data (4)
          RHOA Antibody in Immunocytochemistry (ICC/IF)
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          RHOA Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          RHOA Antibody (66733-1-IG) in ICC/IF

          Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells using 66733-1-IG (RHOA antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IGG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          RHOA Antibody in Immunocytochemistry (ICC/IF)
          RHOA Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RHOA Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RHOA Antibody in Western Blot (WB)
          RHOA Monoclonal Antibody (1B3D7)

          Product Details

          66733-1-IG

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 8 publications 8 publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:500
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Pig, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          1B3D7

          Immunogen

          RHOA Fusion Protein Ag1141 (1-193 aa encoded by BC005976)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • CoraLite 594
          • CoraLite Plus 488
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1.5 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          Immunogen sequence: MAAIRKKLV IVGDGACGKT CLLIVFSKDQ FPEVYVPTVF ENYVADIEVD GKQVELALWD TAGQEDYDRL RPLSYPDTDV ILMCFSIDSP DSLENIPEKW TPEVKHFCPN VPIILVGNKK DLRNDEHTRR ELAKMKQEPV KPEEGRDMAN RIGAFGYMEC SAKTKDGVRE VFEMATRAAL QARRGKKKSG CLVL (1-193 aa encoded by BC005976)

          Target Information

          RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It is involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis, and plays an essential role in cleavage furrow formation. RhoA is required for the apical junction formation of keratinocyte cell-cell adhesion, and serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RhoA stimulates PKN2 kinase activity and may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP. It is essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Aplysia ras-related homolog 12; aplysia ras-related homolog A; aplysia ras-related homolog A1; aplysia ras-related homolog A2; epididymis secretory sperm binding protein; h12; MGC72339; oncogene RHO H12; plysia ras-related homolog A2; Ras family member A; ras homolog A1; ras homolog A2; ras homolog gene family, member A; ras homolog gene family, member A1; ras homolog gene family, member A2; Rho; Rho A; Rho cDNA clone 12; Rho family GTPase; small GTP binding protein RhoA; Transforming protein RhoA; unnamed protein product

          View more View less

          Gene Aliases: ARH12; ARHA; Arha1; Arha2; EDFAOB; RHO12; RHOA; RHOH12

          View more View less

          UniProt ID: (Human) P61586, (Rat) P61589, (Mouse) Q9QUI0

          View more View less

          Entrez Gene ID: (Human) 387, (Pig) 396849, (Rat) 117273, (Mouse) 11848

          View more View less

          Function(s)
          nucleotide binding GTPase activity obsolete small monomeric GTPase activity protein binding GTP binding hydrolase activity myosin binding protein kinase binding GDP binding protein domain specific binding Rho GDP-dissociation inhibitor binding small GTPase
          Process(es)
          mitotic cytokinesis cell morphogenesis neuron migration kidney development angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure alpha-beta T cell lineage commitment regulation of systemic arterial blood pressure by endothelin aortic valve formation regulation of transcription from RNA polymerase II promoter cytoskeleton organization cell adhesion cell-matrix adhesion small GTPase mediated signal transduction Rho protein signal transduction skeletal muscle tissue development negative regulation of cell-substrate adhesion regulation of neuron projection development cell migration substantia nigra development cerebral cortex cell migration forebrain radial glial cell differentiation actin cytoskeleton organization cell differentiation regulation of cell migration positive regulation of cell migration androgen receptor signaling pathway cytoplasmic microtubule organization positive regulation of cytokinesis regulation of actin cytoskeleton organization negative regulation of intracellular steroid hormone receptor signaling pathway regulation of osteoblast proliferation cell junction assembly substrate adhesion-dependent cell spreading Roundabout signaling pathway cleavage furrow formation apolipoprotein A-I-mediated signaling pathway odontogenesis positive regulation of I-kappaB kinase/NF-kappaB signaling stress fiber assembly apical junction assembly beta selection endothelial cell migration ossification involved in bone maturation wound healing, spreading of cells establishment of epithelial cell apical/basal polarity positive regulation of neuron differentiation negative regulation of cell size positive regulation of alpha-beta T cell differentiation negative chemotaxis cell division positive regulation of stress fiber assembly regulation of focal adhesion assembly Wnt signaling pathway, planar cell polarity pathway positive regulation of lipase activity bone trabecula morphogenesis regulation of microtubule cytoskeleton organization cellular response to lipopolysaccharide cellular response to cytokine stimulus semaphorin-plexin signaling pathway positive regulation of podosome assembly positive regulation of protein serine/threonine kinase activity negative regulation of cell migration involved in sprouting angiogenesis mitotic spindle assembly negative regulation of oxidative phosphorylation motor neuron apoptotic process endothelial tube lumen extension regulation of modification of postsynaptic structure positive regulation of NIK/NF-kappaB signaling skeletal muscle satellite cell migration negative regulation of reactive oxygen species biosynthetic process mitotic cleavage furrow formation positive regulation of vascular smooth muscle contraction positive regulation of leukocyte adhesion to vascular endothelial cell regulation of modification of postsynaptic actin cytoskeleton cellular response to chemokine regulation of neural precursor cell proliferation positive regulation of T cell migration negative regulation of motor neuron apoptotic process response to hypoxia regulation of actin polymerization or depolymerization metabolic process response to mechanical stimulus response to glucose positive regulation of cell growth positive regulation of actin filament polymerization stress-activated protein kinase signaling cascade response to drug negative regulation of I-kappaB kinase/NF-kappaB signaling response to amino acid positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of neuron apoptotic process positive regulation of neuron apoptotic process response to ethanol negative regulation of neuron differentiation positive regulation of translation positive regulation of cell adhesion positive regulation of vasoconstriction positive regulation of smooth muscle contraction neuron projection morphogenesis regulation of dendrite development response to glucocorticoid regulation of calcium ion transport negative regulation of cell death trabecula morphogenesis cellular response to progesterone stimulus cell cycle integrin-mediated signaling pathway GTP metabolic process
          It has to be done as per old AB suggested Products section.

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