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          • Primary Antibodies ›
          • ERK1/2 Antibodies

          Invitrogen

          Phospho-ERK1/ERK2 (Thr185, Tyr187) Recombinant Rabbit Monoclonal Antibody (15H10L7)

          Advanced Verification
          4 Published Figures
          16 References
          View all (81) ERK1/2 antibodies

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          Cite Phospho-ERK1/ERK2 (Thr185, Tyr187) Recombinant Rabbit Monoclonal Antibody (15H10L7)

          Additional Information:
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          • Antibody Testing Data (6)
          • Published Figures (4)
          • Advanced Verification (3)
          Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Immunocytochemistry (ICC/IF)
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          Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 13

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          Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody (700012) in ICC/IF

          Immunofluorescence analysis of Phosph-ERK1/2 (Thr 185,Tyr187) was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ERK2 Rabbit monoclonal Antibody (Product # 700012) at 5 µg/mL in 0.1% BSA and incubated over... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Western Blot (WB)
          Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Western Blot (WB)
          Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Western Blot (WB)
          Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Immunohistochemistry (IHC)
          Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Western Blot (WB)
          Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Western Blot (WB)
          Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Western Blot (WB)
          Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody
          Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody
          Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody
          Phospho-ERK1/ERK2 (Thr185, Tyr187) Recombinant Rabbit Monoclonal Antibody (15H10L7)

          Product Details

          700012

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1 µg/mL
          View 13 publications 13 publications

          Immunohistochemistry (IHC)

          -
          View 2 publications 2 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          5 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          -

          ELISA (ELISA)

          Assay-dependent
          -

          ChIP assay (ChIP)

          1 µL
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Dog, Human, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          Expi293

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          15H10L7

          Immunogen

          A peptide corresponding to amino acids 198-208 and 181-191 of P27361 and P28482, respectively.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS

          Contains

          0.09% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Wet ice

          RRID

          AB_2532267

          Product Specific Information

          700012 has successfully been used in western blot to detect phospho-ERK1/2 in TPA-treated HeLa, NRK and MDCK cell lysates.

          This antibody is predicted to react with bovine, chicken, chimpanzee, mouse, rat, Xenopus and zebrafish based on sequence homology.

          Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

          Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

          Target Information

          ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: ERK-1; ERK-2; erk1 erk2; Erk1; cancer susceptibility protein; ERK1b; ERK1c; Erk2; cancer susceptibility protein; ERT1; ERT2; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; Microtubule-associated protein 2 kinase; mitogen-activated 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; MNK1; p42; p42 MAP Kinase; p42-MAPK; p44-ERK1; p44-MAPK; protein tyrosine kinase ERK2; unnamed protein product

          View more View less

          Gene Aliases: ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; Mapk; MAPK1; MAPK2; MAPK3; MNK1; NS13; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3

          View more View less

          UniProt ID: (Human) P28482, (Human) P27361, (Rat) P63086, (Rat) P21708

          View more View less

          Entrez Gene ID: (Human) 5594, (Dog) 477575, (Dog) 100855877, (Human) 5595, (Rat) 116590, (Rat) 50689

          View more View less

          Function(s)
          nucleotide binding phosphotyrosine binding DNA binding protein kinase activity protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity transferase activity phosphatase binding identical protein binding protein serine kinase activity DNA-binding transcription factor binding transcription factor binding protein kinase binding mitogen-activated protein kinase kinase kinase binding scaffold protein binding non-receptor serine/threonine protein kinase
          Process(es)
          MAPK cascade regulation of transcription from RNA polymerase II promoter protein phosphorylation apoptotic process chemotaxis cellular response to DNA damage stimulus signal transduction cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway chemical synaptic transmission heart development learning or memory insulin receptor signaling pathway organ morphogenesis positive regulation of macrophage chemotaxis positive regulation of peptidyl-threonine phosphorylation neural crest cell development Schwann cell development peptidyl-threonine phosphorylation cytosine metabolic process regulation of ossification regulation of cellular pH thyroid gland development regulation of protein stability lipopolysaccharide-mediated signaling pathway positive regulation of telomere maintenance response to lipopolysaccharide regulation of stress-activated MAPK cascade mammary gland epithelial cell proliferation cellular response to amino acid starvation response to nicotine intracellular signal transduction ERBB signaling pathway ERBB2-ERBB3 signaling pathway outer ear morphogenesis myelination response to exogenous dsRNA positive regulation of cholesterol biosynthetic process negative regulation of cell differentiation insulin-like growth factor receptor signaling pathway thymus development T cell receptor signaling pathway B cell receptor signaling pathway stress-activated MAPK cascade regulation of cytoskeleton organization Bergmann glial cell differentiation long-term synaptic potentiation face development lung morphogenesis trachea formation labyrinthine layer blood vessel development cardiac neural crest cell development involved in heart development interleukin-34-mediated signaling pathway chemokine-mediated signaling pathway ERK1 and ERK2 cascade response to epidermal growth factor cellular response to tumor necrosis factor caveolin-mediated endocytosis regulation of Golgi inheritance positive regulation of macrophage proliferation positive regulation of neuroinflammatory response regulation of early endosome to late endosome transport autophagosome assembly negative regulation of T cell mediated immune response to tumor cell transcription, DNA-templated regulation of gene expression negative regulation of autophagy positive regulation of autophagy phosphorylation sensory perception of pain BMP signaling pathway positive regulation of cyclase activity cellular response to nutrient levels negative regulation of proteasomal ubiquitin-dependent protein catabolic process peptidyl-tyrosine autophosphorylation TORC1 signaling glucose homeostasis signal transduction in response to DNA damage positive regulation of transcription from RNA polymerase II promoter modulation of synaptic transmission protein stabilization negative regulation of T cell activation cartilage development positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway cellular response to mechanical stimulus negative regulation of cholesterol efflux xenophagy negative regulation of TORC1 signaling positive regulation of xenophagy MAPK import into nucleus positive regulation of cell proliferation response to toxic substance peptidyl-serine phosphorylation positive regulation of cell migration response to estrogen positive regulation of translation positive regulation of transcription, DNA-templated cellular response to organic substance cellular response to granulocyte macrophage colony-stimulating factor stimulus positive regulation of protein phosphorylation protein complex assembly DNA damage induced protein phosphorylation arachidonic acid metabolic process positive regulation of histone phosphorylation positive regulation of histone acetylation regulation of sequence-specific DNA binding transcription factor activity negative regulation of apolipoprotein binding
          It has to be done as per old AB suggested Products section.

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