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Phospho-ERK1/ERK2 (Thr185, Tyr187) Recombinant Rabbit Monoclonal Antibody (15H10L7)

Advanced Verification
4 Published Figures
16 References
View all (87) ERK1/2 antibodies

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Phospho-ERK1/ERK2 (Thr185, Tyr187) Recombinant Rabbit Monoclonal Antibody (15H10L7)

Product Details

700012

Applications
Tested Dilution
Publications

Western Blot (WB)

1 µg/mL
View 13 publications 13 publications

Immunohistochemistry (IHC)

-
View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-

ELISA (ELISA)

Assay-dependent
-

ChIP assay (ChIP)

1 µL
View 1 publication 1 publication
Product Specifications

Species Reactivity

Dog, Human, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

15H10L7

Immunogen

A peptide corresponding to amino acids 198-208 and 181-191 of P27361 and P28482, respectively.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS, pH 7.2-7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532267

Product Specific Information

700012 has successfully been used in western blot to detect phospho-ERK1/2 in TPA-treated HeLa, NRK and MDCK cell lysates.

This antibody is predicted to react with bovine, chicken, chimpanzee, mouse, rat, Xenopus and zebrafish based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: erk1 erk2; Erk1; cancer susceptibility protein; ERK1; MAP kinase; mitogen-activated protein kinase; ERK1b; ERK1c; Erk2; cancer susceptibility protein; extracellular signal-regulated kinase 1; extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAPK 1; MAPK 2; MAPK 3; microtubule-associated protein 2 kinase; mitogen-activated 3; mitogen-activated protein kinase 1; mitogen-activated protein kinase 2; p42; p42 MAP Kinase; protein serine/threonine kinase ERK1b; protein tyrosine kinase ERK2; unnamed protein product

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Gene Aliases: ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; MAPK1; MAPK2; MNK1; NS13; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3

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UniProt ID: (Human) P28482, (Human) P27361, (Rat) P63086, (Rat) P21708

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Entrez Gene ID: (Human) 5594, (Dog) 477575, (Dog) 100855877, (Human) 5595, (Rat) 116590, (Rat) 50689

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Function(s)
phosphotyrosine binding protein kinase activity protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity phosphatase binding identical protein binding protein serine kinase activity DNA-binding transcription factor binding double-stranded DNA binding protein kinase binding mitogen-activated protein kinase kinase kinase binding scaffold protein binding non-receptor serine/threonine protein kinase
Process(es)
MAPK cascade regulation of transcription from RNA polymerase II promoter protein phosphorylation apoptotic process chemotaxis signal transduction cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway chemical synaptic transmission learning or memory insulin receptor signaling pathway positive regulation of macrophage chemotaxis positive regulation of peptidyl-threonine phosphorylation Schwann cell development positive regulation of telomere maintenance regulation of stress-activated MAPK cascade cellular response to amino acid starvation response to nicotine intracellular signal transduction ERBB signaling pathway ERBB2-ERBB3 signaling pathway myelination positive regulation of skeletal muscle tissue regeneration positive regulation of cholesterol biosynthetic process positive regulation of smoothened signaling pathway insulin-like growth factor receptor signaling pathway stress-activated MAPK cascade regulation of cytoskeleton organization interleukin-34-mediated signaling pathway chemokine-mediated signaling pathway ERK1 and ERK2 cascade response to epidermal growth factor caveolin-mediated endocytosis regulation of Golgi inheritance positive regulation of macrophage proliferation positive regulation of neuroinflammatory response regulation of early endosome to late endosome transport negative regulation of T cell mediated immune response to tumor cell phosphorylation sensory perception of pain BMP signaling pathway positive regulation of cyclase activity peptidyl-tyrosine autophosphorylation signal transduction in response to DNA damage positive regulation of transcription from RNA polymerase II promoter modulation of synaptic transmission positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway cellular response to mechanical stimulus negative regulation of cholesterol efflux negative regulation of TORC1 signaling cellular response to DNA damage stimulus heart development positive regulation of cell proliferation response to toxic substance organ morphogenesis neural crest cell development diadenosine tetraphosphate biosynthetic process peptidyl-serine phosphorylation cytosine metabolic process regulation of ossification positive regulation of cell migration thyroid gland development lipopolysaccharide-mediated signaling pathway response to estradiol response to lipopolysaccharide response to testosterone mammary gland epithelial cell proliferation cellular response to platelet-derived growth factor stimulus response to cocaine positive regulation of protein import into nucleus outer ear morphogenesis response to hydrogen peroxide response to exogenous dsRNA response to estrogen estrous cycle negative regulation of cell differentiation positive regulation of translation positive regulation of transcription, DNA-templated decidualization thymus development T cell receptor signaling pathway B cell receptor signaling pathway regulation of intracellular pH Bergmann glial cell differentiation positive regulation of cardiac muscle cell proliferation long-term synaptic potentiation face development lung morphogenesis trachea formation labyrinthine layer blood vessel development cardiac neural crest cell development involved in heart development cellular response to methionine cellular response to cAMP cellular response to tumor necrosis factor cellular response to epidermal growth factor stimulus cellular response to peptide hormone stimulus cellular response to prostaglandin E stimulus cellular response to lipid cellular response to toxic substance response to alcohol cellular response to insulin-like growth factor stimulus arachidonic acid metabolic process cartilage development positive regulation of xenophagy
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