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Invitrogen
IRAK4 Recombinant Rabbit Monoclonal Antibody (12H2L6)
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FIGURE: 1 / 6
IRAK4 Antibody (700026) in ICC/IF
Immunofluorescent analysis of IRAK-4 in HeLa cells using an IRAK-4 recombinant rabbit monoclonal antibody (Product # 700026) at a dilution of 5 µg/mL followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Actin was stained using Alexa Fluor 568 Phalloidin at a dilution of 1:200. Panels: Hoechst only (top left), AF488 signal only (top right) and composite image with Phalloidin (bottom left).
Product Details
700026
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
12H2L6
Immunogen
A peptide corresponding to amino acids 41-52 of Q9NWZ3.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with mouse, rat, primate, ovine, equine, porcine, bovine, canine and Xenopus based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. Overexpression of IRAK-4 activates NF-kappa-B and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: interleukin-1; Interleukin-1 receptor-associated kinase 4; IRAK-4; IRAK-4 mutated form 1; IRAK-4 mutated form 2; IRAK4; protein kinase; IRAK4S; protein kinase; protein kinase; Renal carcinoma antigen NY-REN-64; unnamed protein product
Gene Aliases: IMD67; IPD1; IRAK-4; IRAK4; NY-REN-64; REN64
UniProt ID: (Human) Q9NWZ3
Entrez Gene ID: (Human) 51135
toll-like receptor signaling pathway
immune system process
neutrophil mediated immunity
MyD88-dependent toll-like receptor signaling pathway
signal transduction
JNK cascade
Toll signaling pathway
cytokine-mediated signaling pathway
positive regulation of defense response
lipopolysaccharide-mediated signaling pathway
toll-like receptor 4 signaling pathway
toll-like receptor 9 signaling pathway
intracellular signal transduction
NIK/NF-kappaB signaling
interleukin-33-mediated signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
positive regulation of smooth muscle cell proliferation
interleukin-1-mediated signaling pathway
neutrophil migration
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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