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Invitrogen

Phospho-SMAD1/SMAD5 (Ser463, Ser465) Recombinant Rabbit Monoclonal Antibody (31H14L11)

Advanced Verification
1 Published Figure
8 References
View all (5) SMAD1/SMAD5 antibodies

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Phospho-SMAD1/SMAD5 (Ser463, Ser465) Recombinant Rabbit Monoclonal Antibody (31H14L11)

Product Details

700047

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
View 2 publications 2 publications

Immunohistochemistry (IHC)

-
View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

4-6 µg/mL
-

Immunocytochemistry (ICC/IF)

2-4 µg/mL
View 1 publication 1 publication

Flow Cytometry (Flow)

0.5-5 µg/1x10^6 cells
-

Miscellaneous PubMed (Misc)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Fish, Mammal, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

31H14L11

Immunogen

A peptide corresponding to amino acids 456-465 of Q15797 and Q99717.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532276

Product Specific Information

This antibody is predicted to react with mouse, primate, rat, Rhesus monkey , sea urchin, ovine, porcine, Xenopus and zebrafish based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

The Smad family of proteins are functioning in the transmission of extracellular signals in the TGF-beta signaling pathway. Binding of a TGF-beta superfamily ligands to extracellular receptors triggers phosphorylation of Smad2 at a Serine-Serine-Methionine-Serine (SSMS) motif at its C-terminus. Phosphorylated Smad2 is then able to form a complex with Smad4. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression. In mammals, eight Smad proteins have been identified to date. The Smad family of proteins can be divided into three functional groups: the receptor-activated Smads (R-Smads), common mediator Smads (Co-Smads), and the inhibitory Smads (I-Smads). The R-Smads are directly phosphorylated by the activated type I receptors on their C-terminal Ser-Ser-X-Ser (SSXS) motif and include Smad1, Smad2, Smad3, Smad5, and Smad8. Smad2 and Smad3 are phosphorylated in response to TGF-beta and activin, whereas Smad1, Smad5, and Smad8 are phosphorylated in response to BMP (Bone Morphogenetic Protein). This C-terminal phosphorylation allows R-Smad binding to Co-Smad, Smad4, and translocation to the nucleus where they regulate TGF-beta target genes. Smad6 and Smad7 belong to the I-Smad which bind to the type I receptor or Smad4 and block their interaction with R-Smads.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: BSP-1; chromosome 5 Mad homolog; hSMAD1; hSmad5; JV4-1; JV5-1; MAD homolog 1; MAD homolog 5; MAD, mothers against decapentaplegic homolog 1; MAD, mothers against decapentaplegic homolog 5; Mad-related protein 1; MADH5; Mothers against decapentaplegic homolog 1; Mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; mothers against DPP homolog 1; OTTHUMP00000223331; Sma and Mad homolog; SMA- and MAD-related protein 5; SMAD; SMAD 1; SMAD 5; SMAD family member 1; SMAD family member 5; SMAD, mothers against DPP homolog 1; SMAD, mothers against DPP homolog 5; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1; unnamed protein product

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Gene Aliases: BSP-1; BSP1; DWFC; JV4-1; JV41; JV5-1; MADH1; MADH5; MADR1; SMAD1; SMAD5

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UniProt ID: (Human) Q15797, (Human) Q99717

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Entrez Gene ID: (Human) 4086, (Human) 4090

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding DEAD/H-box RNA helicase binding protein kinase binding ubiquitin protein ligase binding identical protein binding sequence-specific DNA binding metal ion binding co-SMAD binding I-SMAD binding primary miRNA binding RNA polymerase II regulatory region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding sequence-specific double-stranded DNA binding
Process(es)
MAPK cascade ossification osteoblast differentiation ureteric bud development mesodermal cell fate commitment osteoblast fate commitment cardiac conduction system development transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter cellular iron ion homeostasis inflammatory response signal transduction transforming growth factor beta receptor signaling pathway gamete generation cell proliferation negative regulation of cell proliferation anatomical structure morphogenesis embryonic pattern specification positive regulation of gene expression cell differentiation BMP signaling pathway midbrain development hindbrain development primary miRNA processing developmental process homeostatic process positive regulation of osteoblast differentiation positive regulation of transcription from RNA polymerase II promoter stem cell differentiation negative regulation of muscle cell differentiation cartilage development cardiac muscle cell proliferation bone development SMAD protein signal transduction positive regulation of cartilage development cellular response to growth factor stimulus transforming growth factor beta receptor superfamily signaling pathway positive regulation of dendrite development positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of sprouting angiogenesis anti-Mullerian hormone signaling pathway negative regulation of transcription from RNA polymerase II promoter Mullerian duct regression germ cell development negative regulation of gene expression erythrocyte differentiation negative regulation of apoptotic process positive regulation of transcription, DNA-templated cardiac muscle contraction negative regulation of Fas signaling pathway
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