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Invitrogen

PYK2 Recombinant Rabbit Monoclonal Antibody (9H12L1)

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2 Published Figures
2 References
View all (39) PYK2 antibodies

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PYK2 Recombinant Rabbit Monoclonal Antibody (9H12L1)

Product Details

700183

Applications
Tested Dilution
Publications

Western Blot (WB)

1-3 µg/mL
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

4-6 µg/mL
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-

Flow Cytometry (Flow)

2-4 µg/1x10^6 cells
View 1 publication 1 publication

ChIP assay (ChIP)

1 µL
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

9H12L1

Immunogen

A recombinant protein corresponding to amino acids 780-880 of Q14289.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532294

Product Specific Information

This antibody is predicted to react with bovine, canine, chimpanzee, equine, mouse, orangutan, rat and Rhesus monkey based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

PTK2B (FAK2) is a cytoplasmic tyrosine kinase involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. PTK2B regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading, and bone remodeling. FAK2 plays a role in the regulation of the humoral immune response and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. It functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors, cytokine, chemokine, and growth factor receptors, and mediates responses to cellular stress. Aberrant FAK2 expression may play a role in cancer cell proliferation, migration, and invasion in tumor formation and metastasis. Elevated expression has been seen in gliomas, hepatocellular carcinoma, lung cancer, and breast cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CADTK; CAK-beta; Calcium-dependent tyrosine kinase; Calcium-regulated non-receptor proline-rich tyrosine kinase; Cell adhesion kinase beta; FADK; FADK 2; FAK; FAK1; Focal adhesion kinase 2; FRNK; pp125FAK; Proline-rich tyrosine kinase 2; protein kinase B; Protein-tyrosine kinase 2-beta; PTK2B protein tyrosine kinase 2 beta; RAFTK; Related adhesion focal tyrosine kinase; unnamed protein product

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Gene Aliases: CADTK; CAKB; FADK2; FAK2; PKB; PTK; PTK2B; PYK2; RAFTK

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UniProt ID: (Human) Q14289

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Entrez Gene ID: (Human) 2185

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Function(s)
nucleotide binding protein kinase activity calmodulin-dependent protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein binding ATP binding kinase activity transferase activity receptor activator activity ionotropic glutamate receptor binding neurotransmitter receptor regulator activity
Process(es)
angiogenesis neuron migration regulation of cell-matrix adhesion positive regulation of cell-matrix adhesion sprouting angiogenesis adaptive immune response marginal zone B cell differentiation immune system process protein phosphorylation apoptotic process cellular defense response cytoskeleton organization Golgi organization signal transduction cell surface receptor signaling pathway signal complex assembly epidermal growth factor receptor signaling pathway integrin-mediated signaling pathway positive regulation of cell proliferation negative regulation of cell proliferation regulation of cell shape positive regulation of signal transduction positive regulation of endothelial cell migration regulation of macrophage chemotaxis positive regulation of neuron projection development peptidyl-tyrosine phosphorylation regulation of cell adhesion positive regulation of cell migration negative regulation of bone mineralization positive regulation of actin filament polymerization cortical cytoskeleton organization regulation of actin cytoskeleton organization tumor necrosis factor-mediated signaling pathway ionotropic glutamate receptor signaling pathway peptidyl-tyrosine autophosphorylation activation of Janus kinase activity negative regulation of apoptotic process negative regulation of potassium ion transport negative regulation of neuron apoptotic process bone resorption negative regulation of myeloid cell differentiation positive regulation of angiogenesis positive regulation of protein kinase activity positive regulation of JNK cascade protein autophosphorylation vascular endothelial growth factor receptor signaling pathway regulation of synaptic plasticity regulation of multicellular organismal process regulation of release of sequestered calcium ion into cytosol positive regulation of protein kinase B signaling positive regulation of synaptic transmission, glutamatergic long-term synaptic potentiation protein-containing complex assembly chemokine-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade cellular response to retinoic acid cellular response to fluid shear stress endothelin receptor signaling pathway regulation of postsynaptic density assembly regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of excitatory postsynaptic potential positive regulation of B cell chemotaxis
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