Phospho-AKT pSer473 Antibody (98H9L8), ABfinity™ Rabbit Monoclonal

Catalog number: 700392

Invitrogen™  Related applications: Cellular Imaging | Flow Cytometry | Protein Assays and Analysis

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This antibody is predicted to react with bovine, canine, chicken, chimpanzee, equine, feline, rat, Rhesus monkey , Xenopus and zebrafish based on sequence homology.

ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.

ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures.


Reactivity: Human, Mouse
Validated Application: Flow Cytometry, Western Blot, Immunofluorescence, Immunohistochemistry
Host Species: Rabbit
SubClass (Isotype): IgG
Clonality: Monoclonal
Clone ID: 98H9L8
Antibody Fragment: Whole Antibody
Target Gene: AKT [pS473]
Gene ID: 207
Accession Number: P31749
Label or Dye: Unconjugated
Target Modification State: Phospho
Purification: Affinity Purified
Concentration: 0.5 mg⁄ml
Color: Unconjugated
Form: Liquid
Neutralizing Antibody: No
For Use With (Equipment): Fluorescence Microscope, Confocal Microscope, Flow Cytometer
Product Size: 100 µg
Quantity: 1
Volume (Metric): 200 µl
Shipping Condition: Wet Ice

Contents & storage

2-8°C for up to 1 month, -20°C for long term storage. Avoid repeated freezing and thawing.


Manuals & protocols

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