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Invitrogen
Phospho-IGF1R beta (Tyr1135, Tyr1136) Recombinant Rabbit Monoclonal Antibody (15H10L8)
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FIGURE: 1 / 1
Phospho-IGF1R beta (Tyr1135, Tyr1136) Antibody (701067) in WB
Western blot analysis of Phospho-IGF1R pTyr1135/1136 in whole cell extracts of HeLa cells treated with Insulin (100 ng/mL, 15 min) using a Phospho-IGF1R pTyr1135/1136 recombinant rabbit monoclonal antibody (Product # 701067) at a dilution of 2.5 µg/mL. To confirm specificity, competition was performed by preincubation with the phosphopeptide to inhibit antibody binding (lane 1). Results show a band at ~90 k... View More
Product Details
701067
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
15H10L8
Immunogen
Phosphopeptide corresponding to amino acids 1131-1140 of human IGF1R
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
IGF-1R (Insulin-like Growth Factor-1 Receptor) consists of alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD221; IGF-I receptor; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor
Gene Aliases: CD221; IGF1R; IGFIR; IGFR; JTK13
UniProt ID: (Human) P08069
Entrez Gene ID: (Human) 3480
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
insulin-activated receptor activity
insulin-like growth factor-activated receptor activity
insulin receptor binding
structural molecule activity
protein binding
insulin-like growth factor binding
ATP binding
kinase activity
transferase activity
insulin-like growth factor I binding
identical protein binding
phosphatidylinositol 3-kinase binding
insulin binding
insulin receptor substrate binding
protein transporter activity
immune response
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of cell proliferation
insulin receptor signaling pathway
positive regulation of cell migration
peptidyl-tyrosine autophosphorylation
negative regulation of apoptotic process
positive regulation of protein complex disassembly
negative regulation of MAPK cascade
positive regulation of MAPK cascade
protein kinase B signaling
transcytosis
regulation of JNK cascade
protein autophosphorylation
insulin-like growth factor receptor signaling pathway
positive regulation of protein kinase B signaling
cellular response to glucose stimulus
dendritic spine maintenance
beta-amyloid clearance
positive regulation of cold-induced thermogenesis
cellular response to oxygen-containing compound
cellular response to beta-amyloid
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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