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Invitrogen

FAK Recombinant Rabbit Monoclonal Antibody (5H18L19)

2 Published Figures
3 References
View all (105) FAK antibodies

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FAK Recombinant Rabbit Monoclonal Antibody (5H18L19)

Product Details

701094

Applications
Tested Dilution
Publications

Western Blot (WB)

1-3 µg/mL
View 2 publications 2 publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2-4 µg
-
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

5H18L19

Immunogen

Recombinant protein corresponding to amino acids 853-1052 of human FAK1
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532384

Product Specific Information

This antibody is predicted to react with mouse based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: EC 2.7.10.2; FADK 1; FAK-Del33; exon 33 deletion splicing mutant; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; Focal adhesion kinase-related nonkinase; FRNK; p125FAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2; unnamed protein product

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Gene Aliases: FADK; FADK 1; FAK; FAK1; FRNK; p125FAK; pp125FAK; PPP1R71; PTK2

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UniProt ID: (Human) Q05397

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Entrez Gene ID: (Human) 5747

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Function(s)
nucleotide binding actin binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein tyrosine phosphatase activity integrin binding protein binding ATP binding JUN kinase binding kinase activity transferase activity protein kinase binding protein phosphatase binding SH2 domain binding molecular function activator activity
Process(es)
angiogenesis placenta development regulation of protein phosphorylation positive regulation of protein phosphorylation regulation of cell-matrix adhesion heart morphogenesis signal complex assembly epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway axon guidance positive regulation of cell proliferation regulation of cell shape regulation of endothelial cell migration regulation of epithelial cell migration positive regulation of epithelial cell migration positive regulation of epithelial to mesenchymal transition positive regulation of macrophage chemotaxis positive regulation of fibroblast migration cell migration peptidyl-tyrosine phosphorylation negative regulation of cell-cell adhesion establishment of cell polarity cell differentiation regulation of cell adhesion positive regulation of cell migration regulation of cell adhesion mediated by integrin detection of muscle stretch netrin-activated signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis regulation of cell proliferation negative regulation of apoptotic process regulation of GTPase activity regulation of cell differentiation regulation of osteoblast differentiation positive regulation of protein kinase activity protein autophosphorylation vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway cell motility regulation of multicellular organismal process regulation of cytoskeleton organization regulation of focal adhesion assembly positive regulation of protein kinase B signaling growth hormone receptor signaling pathway positive regulation of wound healing vascular endothelial cell response to oscillatory fluid shear stress positive regulation of macrophage proliferation regulation of substrate adhesion-dependent cell spreading positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of anoikis
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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