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Invitrogen
PDGFRA Recombinant Rabbit Monoclonal Antibody (7H13L1)
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FIGURE: 1 / 3
PDGFRA Antibody (701142) in ICC/IF
Immunofluorescent analysis of PDGFR alpha in HeLa cells using a PDGFR alpha recombinant rabbit monoclonal antibody (Product # 701142) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing cytoplasmic and nuclear membrane localization of PDGFR-alpha.
Product Details
701142
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
7H13L1
Immunogen
Recombinant protein corresponding to amino acids 259-436 of human platelet-derived growth factor receptor - alpha polypeptide
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
PDGFRA is a cell surface receptor tyrosine kinase which binds members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a; CD140a antigen; MGC74795; OTTHUMP00000218656; PDGF A-chain; PDGF Receptor alpha; PDGF subunit A; PDGF-1; PDGF-R-alpha; PDGFR-2; pdgfr-a; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2; Platelet-derived growth factor receptor alpha; platelet-derived growth factor receptor, alpha polypeptide; unnamed protein product
Gene Aliases: CD140A; PDGFR-2; PDGFR2; PDGFRA; RHEPDGFRA
UniProt ID: (Human) P16234
Entrez Gene ID: (Human) 5156
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
platelet-derived growth factor alpha-receptor activity
vascular endothelial growth factor-activated receptor activity
platelet-derived growth factor receptor binding
protein binding
ATP binding
kinase activity
transferase activity
vascular endothelial growth factor binding
protein homodimerization activity
macromolecular complex binding
platelet-derived growth factor binding
phospholipase C activator activity
luteinization
in utero embryonic development
cell activation
hematopoietic progenitor cell differentiation
chemotaxis
transmembrane receptor protein tyrosine kinase signaling pathway
estrogen metabolic process
positive regulation of cell proliferation
female gonad development
anatomical structure morphogenesis
organ morphogenesis
negative regulation of platelet activation
cell migration
peptidyl-tyrosine phosphorylation
signal transduction involved in regulation of gene expression
extracellular matrix organization
lung development
adrenal gland development
positive regulation of cell migration
male genitalia development
regulation of actin cytoskeleton organization
Leydig cell differentiation
cellular response to reactive oxygen species
platelet-derived growth factor receptor-alpha signaling pathway
positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway
wound healing
odontogenesis of dentin-containing tooth
protein autophosphorylation
platelet-derived growth factor receptor signaling pathway
positive regulation of fibroblast proliferation
embryonic digestive tract morphogenesis
embryonic cranial skeleton morphogenesis
embryonic skeletal system morphogenesis
skeletal system morphogenesis
positive regulation of calcium-mediated signaling
white fat cell differentiation
positive regulation of chemotaxis
positive regulation of protein kinase B signaling
cardiac myofibril assembly
palate development
face morphogenesis
cell chemotaxis
retina vasculature development in camera-type eye
positive regulation of ERK1 and ERK2 cascade
platelet aggregation
cellular response to amino acid stimulus
metanephric glomerular capillary formation
regulation of mesenchymal stem cell differentiation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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