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Invitrogen
PAX3 Recombinant Rabbit Monoclonal Antibody (16H22L10)
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FIGURE: 1 / 5
PAX3 Antibody (701147) in ICC/IF
Immunofluorescent analysis of PAX3 in U2OS cells using a PAX3 recombinant rabbit monoclonal antibody (Product # 701147) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing nuclear localization of Pax 3.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
701147
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
16H22L10
Immunogen
Recombinant protein corresponding to amino acids 34-276 of human Pax 3
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Pax 3 (Paired Box 3) is a transcription factor that is involved in development of the peripheral nervous system, melanocytes, some vascular smooth muscle, and a number of other derivatives. It regulates neurogenesis in pre-migratory neural crest cells from the dorsal neural tube, and in myogenic progenitors in the presomitic mesoderm and the hypaxial somites. Alternative splicing results in isoforms having different C-termini.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: MGC120381; MGC120382; MGC120383; MGC120384; MGC134778; paired box gene 3; paired box homeotic gene 3; paired domain gene 3; paired domain gene HuP2; PAX-3; PAX3/FKHR fusion; transcription factor; transcriptional factor PAX3; translocation-mediated chimeric transcription factor; unnamed protein product
Gene Aliases: CDHS; HUP2; PAX-3; Sp; Splchl2; splotch; WS1; WS3
UniProt ID: (Human) P23760, (Mouse) P24610
Entrez Gene ID: (Human) 5077, (Mouse) 18505
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
sequence-specific double-stranded DNA binding
transcription cofactor binding
chromatin binding
HMG box domain binding
negative regulation of transcription from RNA polymerase II promoter
neural crest cell migration
neural tube closure
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
nervous system development
heart development
muscle organ development
cell proliferation
positive regulation of cell proliferation
regulation of somitogenesis
cell migration
spinal cord association neuron differentiation
neural tube development
skeletal muscle cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
developmental pigmentation
neuron fate commitment
tissue morphogenesis
cardiac muscle cell differentiation
skeletal muscle organ development
mammary gland specification
apoptotic process
sensory perception of sound
anatomical structure morphogenesis
organ morphogenesis
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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