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Invitrogen
Nkx2.5 Recombinant Rabbit Monoclonal Antibody (4H5L9)
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FIGURE: 1 / 3
Nkx2.5 Antibody (701622) in ICC/IF
Immunocytochemistry analysis of Human cardiomyocytes stained with Nkx2.5 Recombinant Rabbit Monoclonal Antibody (Product # 701622, 5.0 µg/mL) using a: Alexa Fluor® 488 Goat anti-Rabbit as a secondary antibody (green). b: DAPI stained Primary human cardiomyocytes nuclei (blue). c: Actin stained with Alexa Fluor® 594 Phalloidin (red). d: composite image of cells showing nuclear localization. e: No primary control.
Product Details
701622
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
4H5L9
Immunogen
human Nkx2.5
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cardiac-specific homeobox; cardiac-specific homeobox 1; FLJ52202; FLJ97166; FLJ97195; FLJ97197; FLJ99536; Homeobox protein CSX; Homeobox protein NK-2 homolog E; homeobox protein NKX 2-5; Homeobox protein Nkx-2.5; NK2 transcription factor related locus 5 (Drosophila) isoform NKX2-5_1; NK2 transcription factor related locus 5 (Drosophila) isoform NKX2-5_2; NK2 transcription factor related, locus 5; NKX 2-5; Nkx-2.5; Nkx-25; tinman homolog; tinman paralog; unnamed protein product
Gene Aliases: CHNG5; CSX; CSX1; HLHS2; NKX2-5; NKX2.5; NKX2E; NKX4-1; VSD3
UniProt ID: (Human) P52952
Entrez Gene ID: (Human) 1482
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein homodimerization activity
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
vasculogenesis
heart looping
heart morphogenesis
outflow tract septum morphogenesis
outflow tract morphogenesis
cardiac conduction system development
atrioventricular node development
bundle of His development
Purkinje myocyte differentiation
aortic valve morphogenesis
cardiac ventricle morphogenesis
cardiac ventricle formation
right ventricular cardiac muscle tissue morphogenesis
ventricular trabecula myocardium morphogenesis
atrial cardiac muscle tissue development
apoptotic process involved in heart morphogenesis
septum secundum development
proepicardium development
pulmonary myocardium development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
apoptotic process
heart development
adult heart development
cell proliferation
positive regulation of cell proliferation
positive regulation of gene expression
negative regulation of cardiac muscle cell apoptotic process
positive regulation of sodium ion transport
negative regulation of myotube differentiation
hemopoiesis
cell differentiation
epithelial cell differentiation
thyroid gland development
embryonic heart tube development
negative regulation of apoptotic process
positive regulation of neuron differentiation
positive regulation of heart contraction
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
spleen development
cardiac muscle tissue development
epithelial cell proliferation
positive regulation of epithelial cell proliferation
positive regulation of cardioblast differentiation
ventricular cardiac myofibril assembly
cardiac muscle cell differentiation
cardiac muscle tissue morphogenesis
cardiac muscle cell development
atrial cardiac muscle cell development
ventricular cardiac muscle cell development
regulation of cardiac muscle contraction
pharyngeal system development
cardiac muscle cell proliferation
regulation of cardiac muscle cell proliferation
heart contraction
cardiac muscle contraction
positive regulation of transcription initiation from RNA polymerase II promoter
heart trabecula formation
cardiac septum morphogenesis
ventricular septum morphogenesis
atrial septum morphogenesis
atrioventricular node cell development
atrioventricular node cell fate commitment
embryonic heart tube left/right pattern formation
negative regulation of canonical Wnt signaling pathway
regulation of cardiac conduction
epithelial cell apoptotic process
negative regulation of epithelial cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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