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          • Primary Antibodies ›
          • SOX9 Antibodies

          Invitrogen

          SOX9 Recombinant Rabbit Monoclonal Antibody (7H13L8)

          Advanced Verification
          3 Published Figures
          7 References
          View all (64) SOX9 antibodies

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          Cite SOX9 Recombinant Rabbit Monoclonal Antibody (7H13L8)

          Additional Information:
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          • Antibody Testing Data (6)
          • Published Figures (3)
          • Advanced Verification (3)
          SOX9 Antibody in Immunocytochemistry (ICC/IF)
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          SOX9 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 12

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          SOX9 Antibody (702016) in ICC/IF

          For immunofluorescence analysis, HCT116 cells were fixed and permeabilized for detection of endogenous SOX9 using Anti- SOX9 Recombinant Rabbit Monoclonal Antibody (Product # 702016, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of SOX9 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S3693... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          SOX9 Antibody in Immunocytochemistry (ICC/IF)
          SOX9 Antibody in Immunocytochemistry (ICC/IF)
          SOX9 Antibody in Western Blot (WB)
          SOX9 Antibody in Western Blot (WB)
          SOX9 Antibody in Western Blot (WB)
          SOX9 Antibody in ChIP Assay (ChIP)
          SOX9 Antibody in Immunohistochemistry (IHC)
          SOX9 Antibody in Immunohistochemistry (IHC)
          SOX9 Antibody in Western Blot (WB)
          SOX9 Antibody
          SOX9 Antibody
          SOX9 Antibody
          SOX9 Recombinant Rabbit Monoclonal Antibody (7H13L8)

          Product Details

          702016

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          2.5 µg/mL
          View 3 publications 3 publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:100
          View 1 publication 1 publication

          ChIP assay (ChIP)

          2.5 µg/1x10^6 cells
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Expression System

          Expi293

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          7H13L8

          Immunogen

          Peptides corresponding to human SOX9 [1) aa4-aa17 2) aa47-aa62]
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4

          Contains

          0.09% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Wet ice

          RRID

          AB_2716879

          Product Specific Information

          This antibody is predicted to react with Monkey, Pig, Mouse

          Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

          Target Information

          SOX9 has a role in sex determination and differentiation of Sertoli cells. It is involved in chondrogenesis and regulates the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. Translocation of this gene can cause campomelic dysplasia. SOX9 is involved in the formation of testes from the indifferent fetal gonads. It is a major molecular component of the neuron-glia switch in developing spinal cord. During normal development, SOX9 allows the prostate epithelium to outgrow into the mesenchyme and then provides basal cell support for development and maintenance of the luminal epithelium. These functions of SOX9 are subverted in prostate cancer to support tumor growth and invasion. SOX9 may direct the formation of neural crest precursors and the development of a range of neural crest derivative. It has a transcriptional regulation in melanin production in cells. Northern blot analysis shows its expression on adult testis, adult heart, and fetal brain.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: mSox9; SOX 9; SOX9; SRY (sex determining region Y)-box9; SRY (sex-determining region Y)-box 9 protein; SRY-box 9; SRY-Box 9 ; SRY-box containing gene 9; SRY-related HMG-box, gene 9; Transcription factor SOX-9; unnamed protein product

          View more View less

          Gene Aliases: 2010306G03Rik; AV220920; CMD1; CMPD1; ENH13; mKIAA4243; Sox-9; SOX9; SRA1; SRXX2; SRXY10; TES; TESCO

          View more View less

          UniProt ID: (Human) P48436, (Mouse) Q04887

          View more View less

          Entrez Gene ID: (Human) 6662, (Mouse) 20682

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding beta-catenin binding protein kinase A catalytic subunit binding bHLH transcription factor binding sequence-specific DNA binding pre-mRNA intronic binding sequence-specific double-stranded DNA binding core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding enhancer sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein kinase activity enhancer binding transcription regulatory region DNA binding protein heterodimerization activity
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter skeletal system development cartilage condensation cell fate specification epithelial to mesenchymal transition tissue homeostasis positive regulation of protein phosphorylation hair follicle development positive regulation of mesenchymal cell proliferation chondrocyte development negative regulation of immune system process heart valve development heart valve morphogenesis heart valve formation endocardial cushion morphogenesis chondrocyte differentiation involved in endochondral bone morphogenesis chondrocyte hypertrophy nucleosome assembly chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter protein complex assembly protein phosphorylation cytoskeleton organization signal transduction epidermal growth factor receptor signaling pathway Notch signaling pathway spermatogenesis heart development positive regulation of cell proliferation negative regulation of cell proliferation male gonad development regulation of cell cycle process positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial cell migration neural crest cell development positive regulation of phosphatidylinositol 3-kinase signaling single organismal cell-cell adhesion male germ-line sex determination cAMP-mediated signaling cell differentiation regulation of cell adhesion extracellular matrix organization male sex determination negative regulation of ossification negative regulation of bone mineralization negative regulation of epithelial cell differentiation positive regulation of epithelial cell differentiation otic vesicle formation endocrine pancreas development negative regulation of chondrocyte differentiation positive regulation of chondrocyte differentiation lacrimal gland development protein localization to nucleus somatic stem cell population maintenance intrahepatic bile duct development regulation of cell proliferation negative regulation of apoptotic process protein kinase B signaling cell fate commitment regulation of cell differentiation negative regulation of myoblast differentiation positive regulation of protein catabolic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of photoreceptor cell differentiation oligodendrocyte differentiation homeostasis of number of cells within a tissue positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation cartilage development Sertoli cell differentiation Sertoli cell development astrocyte fate commitment retina development in camera-type eye retinal rod cell differentiation endochondral bone morphogenesis epithelial tube branching involved in lung morphogenesis lung epithelial cell differentiation prostate gland morphogenesis epithelial cell proliferation involved in prostatic bud elongation bronchus cartilage development trachea cartilage development intestinal epithelial structure maintenance regulation of cell proliferation involved in tissue homeostasis positive regulation of cartilage development regulation of branching involved in lung morphogenesis morphogenesis of a branching epithelium lung smooth muscle development negative regulation of biomineral tissue development ERK1 and ERK2 cascade Harderian gland development cellular response to mechanical stimulus cellular response to retinoic acid cellular response to epidermal growth factor stimulus cellular response to heparin cellular response to transforming growth factor beta stimulus renal vesicle induction ureter development ureter smooth muscle cell differentiation ureter morphogenesis metanephric nephron tubule formation negative regulation of canonical Wnt signaling pathway cochlea morphogenesis positive regulation of kidney development positive regulation of branching involved in ureteric bud morphogenesis positive regulation of extracellular matrix assembly positive regulation of male gonad development positive regulation of cell proliferation involved in heart morphogenesis positive regulation of mesenchymal stem cell differentiation regulation of epithelial cell proliferation involved in lung morphogenesis negative regulation of mesenchymal cell apoptotic process ossification branching involved in ureteric bud morphogenesis morphogenesis of an epithelium chondrocyte differentiation glandular epithelial cell differentiation aortic valve morphogenesis growth plate cartilage chondrocyte growth apoptotic process central nervous system development cell proliferation mesenchymal cell proliferation gene expression regulation of gene expression neural crest cell fate specification glial cell fate specification bone mineralization prostate gland development mammary gland development notochord development regulation of apoptotic process negative regulation of osteoblast differentiation negative regulation of fatty acid oxidation neuron fate specification epithelial cell proliferation canonical Wnt signaling pathway limb bud formation Type I pneumocyte differentiation intestinal epithelial cell differentiation cell proliferation involved in heart morphogenesis protein-containing complex assembly response to fatty acid cellular response to interleukin-1 otic vesicle development cellular response to BMP stimulus stem cell proliferation metanephric tubule development ureter urothelium development extracellular matrix assembly anterior head development mesenchymal cell apoptotic process cell-cell adhesion positive regulation of chondrocyte proliferation negative regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of beta-catenin-TCF complex assembly positive regulation of stem cell proliferation
          It has to be done as per old AB suggested Products section.

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