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Invitrogen
14-3-3 epsilon Recombinant Rabbit Monoclonal Antibody (5H10L5)
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FIGURE: 1 / 4
14-3-3 epsilon Antibody (702241) in ICC/IF
Immunofluorescence was performed on methanol fixed Hep G2 cells for detection of endogenous 14-3-3 protein epsilon using 14-3-3 protein epsilon/YWHAE Recombinant Rabbit Monoclonal Antibody (Product # 702241, 2µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
702241
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
5H10L5
Immunogen
Peptides corresponding to Human YWHAE (aa 70-87, 238-255)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Sheep, Rat and Mouse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
14-3-3 epsilon belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 14-3-3 epsilon; 14-3-3E; epididymis luminal protein 2; mitochondrial import stimulation factor (MSF) L subunit; mitochondrial import stimulation factor L subunit; MSF L; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein, epsilon polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide; unnamed protein product
Gene Aliases: 14-3-3E; HEL2; KCIP-1; MDCR; MDS
UniProt ID: (Human) P62258, (Mouse) P62259, (Rat) P62260
Entrez Gene ID: (Human) 7531, (Mouse) 22627, (Rat) 29753
RNA binding
protein phosphatase inhibitor activity
calcium channel regulator activity
protein binding
potassium channel regulator activity
calcium channel inhibitor activity
enzyme binding
protein phosphatase binding
protein domain specific binding
MHC class II protein complex binding
ubiquitin protein ligase binding
signaling adaptor activity
identical protein binding
histone deacetylase binding
ion channel binding
cadherin binding
protein heterodimerization activity
phosphoserine binding
phosphoprotein binding
scaffold protein binding
protein sequestering activity
molecular function inhibitor activity
enzyme inhibitor activity
macromolecular complex binding
protein kinase C inhibitor activity
MAPK cascade
cytoplasmic pattern recognition receptor signaling pathway
regulation of heart rate by hormone
signal transduction
regulation of mitotic cell cycle
protein localization
substantia nigra development
cellular potassium ion homeostasis
negative regulation of toll-like receptor signaling pathway
protein localization to nucleus
cellular response to heat
positive regulation of hippo signaling
intracellular signal transduction
negative regulation of protein import into nucleus
positive regulation of protein export from nucleus
regulation of cytosolic calcium ion concentration
regulation of membrane repolarization
protein localization to endoplasmic reticulum
membrane repolarization during cardiac muscle cell action potential
regulation of heart rate by cardiac conduction
regulation of potassium ion transmembrane transport
negative regulation of calcium ion export across plasma membrane
neuron migration
protein targeting
ER to Golgi vesicle-mediated transport
Golgi to plasma membrane transport
hippocampus development
cerebral cortex development
positive regulation of toll-like receptor signaling pathway
toll-like receptor 4 signaling pathway
hippo signaling
establishment of protein localization
protein K11-linked ubiquitination
regulation of postsynaptic membrane neurotransmitter receptor levels
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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