Search
Invitrogen
PCK2 Recombinant Rabbit Monoclonal Antibody (16H5L22)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 6
PCK2 Antibody (702748) in ICC/IF
For immunofluorescence analysis, HepG2 cells were fixed and permeabilized for detection of endogenous PCK2 using Anti- PCK2 Recombinant Rabbit Monoclonal Antibody (Product # 702748, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of PCK2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938... View More
Product Details
702748
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
16H5L22
Immunogen
Peptides corresponding to human PEPCK [1) aa621-aa637 2) aa395-aa414]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Rabbit, Bovine, Horse
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; HGNC:8725; mtPCK2; PCK2; PEP carboxykinase; PEPCK-M; phosphoenolpyruvate carboxykinase 2 (mitochondrial) isoform PCK2_1; phosphoenolpyruvate carboxykinase 2 (mitochondrial) isoform PCK2_2; phosphoenolpyruvate carboxykinase 2 (mitochondrial) isoform PCK2_3; Phosphoenolpyruvate carboxykinase 2, mitochondrial; Phosphoenolpyruvate carboxykinase [GTP], mitochondrial; phosphopyruvate carboxylase; Serine/threonine-protein kinase PCK2; unnamed protein product
Gene Aliases: mtPCK2; PCK2; PEPCK; PEPCK-M; PEPCK2
UniProt ID: (Human) Q16822
Entrez Gene ID: (Human) 5106
gluconeogenesis
oxaloacetate metabolic process
propionate catabolic process
positive regulation of insulin secretion
response to lipopolysaccharide
cellular response to insulin stimulus
response to starvation
glycerol biosynthetic process from pyruvate
hepatocyte differentiation
cellular response to glucose stimulus
cellular response to tumor necrosis factor
response to dexamethasone
cellular response to dexamethasone stimulus
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support