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Invitrogen
DNM1L Recombinant Rabbit Monoclonal Antibody (2H15L40)
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FIGURE: 1 / 4
DNM1L Antibody (702782) in ICC/IF
For immunofluorescence analysis, SH-SY5Y cells were fixed and permeabilized for detection of endogenous DNM1L using Anti- DNM1L Recombinant Rabbit Monoclonal Antibody (Product # 702782, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000 dilution). Panel a) shows representative cells that were stained for detection and localization of DNM1L protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with D... View More
Product Details
702782
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
2H15L40
Immunogen
Peptides corresponding to human DNM1L [1) aa406-aa423, 2) aa443-aa458]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Pig.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
A human dynamin-related protein, DRP1 contributes to mitochondrial division in mammalian cells. It plays this important role in mitochondrial fission at steady state and during apoptosis. DRP1 is required for proper cellular distribution of mitochondria, and in mutant neurons, mitochondria are largely absent from synapses, thus providing a genetic tool to assess the role of mitochondria at synapses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-terminal region; Dnm1p/Vps1p-like protein; DVLP; Dymple; Dynamin family member proline-rich carboxyl-terminal domain less; Dynamin-1-like protein; Dynamin-like protein; dynamin-like protein 1; Dynamin-like protein 4; Dynamin-like protein IV; Dynamin-related protein 1; DYNIV 11; DYNIV-11; EC 3.6.5.5; FLJ41912; HdynIV; large G-protein; N-terminal region; putative GTP-binding protein; unnamed protein product; VPS1
Gene Aliases: 6330417M19Rik; AI450666; DLP1; DNM1L; Dnml1; Dnmlp1; DRP1; DVLP; DYMPLE; EMPF; EMPF1; HDYNIV; OPA5; python
UniProt ID: (Human) O00429, (Rat) O35303, (Mouse) Q8K1M6
Entrez Gene ID: (Human) 10059, (Rat) 114114, (Mouse) 74006
nucleotide binding
GTPase activity
GTPase activator activity
protein binding
GTP binding
microtubule binding
lipid binding
hydrolase activity
GTP-dependent protein binding
small GTPase binding
ubiquitin protein ligase binding
identical protein binding
protein homodimerization activity
membrane traffic protein
mitochondrial fission
calcium ion transport
endocytosis
mitochondrion organization
endoplasmic reticulum organization
regulation of gene expression
regulation of mitochondrion organization
programmed cell death
peroxisome fission
mitochondrial fragmentation involved in apoptotic process
intracellular distribution of mitochondria
rhythmic process
positive regulation of protein secretion
protein oligomerization
heart contraction
protein localization to mitochondrion
positive regulation of neutrophil chemotaxis
positive regulation of mitochondrial fission
mitochondrial membrane fission
mitocytosis
regulation of peroxisome organization
regulation of mitophagy
positive regulation of secretion by cell
regulation of ATP metabolic process
release of cytochrome c from mitochondria
dynamin polymerization involved in mitochondrial fission
metabolic process
regulation of protein oligomerization
positive regulation of apoptotic process
protein homotetramerization
membrane fusion
necroptotic process
mitochondrion morphogenesis
positive regulation of release of cytochrome c from mitochondria
regulation of mitophagy
positive regulation of intrinsic apoptotic signaling pathway
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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