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Invitrogen
IFI16 Recombinant Rabbit Monoclonal Antibody (8H37L1)
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FIGURE: 1 / 3
IFI16 Antibody (703147) in ICC/IF
For immunofluorescence analysis, THP-1 cells were fixed and permeabilized for detection of endogenous IFI16 using Anti-IFI16 Recombinant Rabbit Monoclonal Antibody (Product # 703147, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of IFI16 protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Produ... View More
Product Details
703147
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
8H37L1
Immunogen
Protein corresponding to human IFI16 [1) aa571-aa766 2) aa446-aa571]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Mouse, Rat, Bovine.
Target Information
IFI16 (gamma-inteferon-inducible protein 16) binds double-stranded DNA. It binds preferentially to supercoiled DNA and cruciform DNA structures. It seems to be involved in transcriptional regulation and as a transcriptional repressor. IFI16 may have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. It controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. IFI16 is also involved in innate immune response by recognizing viral dsDNA in the cytosol and nucleus. After binding to viral DNA in the cytoplasm, IFI16 recruits TMEM173/STING and mediates the induction of IFN-beta. It is necessary to activate the IFR3 signaling cascade suring human herpes simplex virus 1 infection and promotes the assembly of heterochromatin on herpesviral DNA and inhibiton of viral immediate-early gene expression and replication. IFI16 is also involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Gamma-interferon-inducible protein 16; IFI 16C; Ifi-16; IFI16; interferon-gamma induced protein IFI 16; Interferon-inducible myeloid differentiation transcriptional activator; unnamed protein product
Gene Aliases: IFI16; IFNGIP1; PYHIN2
UniProt ID: (Human) Q16666
Entrez Gene ID: (Human) 3428
negative regulation of transcription from RNA polymerase II promoter
positive regulation of cytokine production
activation of innate immune response
immune system process
autophagy
apoptotic process
inflammatory response
regulation of autophagy
myeloid cell differentiation
monocyte differentiation
positive regulation of interleukin-1 beta production
cellular response to interferon-beta
cellular response to glucose starvation
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
negative regulation of DNA binding
negative regulation of viral genome replication
innate immune response
negative regulation of gene expression, epigenetic
negative regulation of innate immune response
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of inflammatory response
defense response to virus
cellular response to ionizing radiation
intrinsic apoptotic signaling pathway by p53 class mediator
negative regulation of AIM2 inflammasome complex assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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