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Invitrogen
NeuroG3 Recombinant Rabbit Monoclonal Antibody (10H35L14)
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FIGURE: 1 / 2
NeuroG3 Antibody (703206) in WB
Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of MDAM-MB-231 (Lane 1), IMR-32 (Lane 2) and tissue extracts of Mouse Pancreas (Lane 3), Mouse Brain (Lane 4) and Mouse Pup Brain (Lane 5). The blot was probed with Anti-Neurogenin-3 Recombinant Rabbit Monoclonal Antibody (Product # 703206, 1:1,000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4,000 dilution). A 30 kDa corresponding... View More
Product Details
703206
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
10H35L14
Immunogen
Protein corresponding to human Neurogenin-3 [aa1-aa214]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Goat, Bovine.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Neurog 3 protein contains one basic helix-loop-helix (bHLH) domain.It is both necessary and sufficient to induce endocrine islet cell differentiation from embryonic pancreatic progenitors. Not only does itinitiates endocrine cell differentiation, italso promotes islet cell maturation and maintains islet function. It is reported that Neurogenin 3 can redirect the differentiation of bipotential secretory progenitors to endocrine rather than goblet cell fate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: atonal homolog 5; bHLHa7; Class A basic helix-loop-helix protein 7; Helix-loop-helix protein mATH-4B; mATH4B; Neurogenin 3 (Atonal protein homolog 5) (Helix-loop-helix protein mATH-4B) (MATH4B); Neurogenin-3; neurogenin3; NGN-3; Protein atonal homolog 5; unnamed protein product
Gene Aliases: Ath4b; ATOH5; BHLHA7; Math4B; NEUROG3; NGN-3; NGN3
UniProt ID: (Human) Q9Y4Z2, (Mouse) P70661
Entrez Gene ID: (Human) 50674, (Mouse) 11925
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
chromatin DNA binding
protein dimerization activity
E-box binding
sequence-specific double-stranded DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription coactivator activity
basic helix-loop-helix transcription factor
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
nervous system development
central nervous system development
peripheral nervous system development
sensory organ development
spinal cord development
cell differentiation
epithelial cell differentiation
forebrain development
hindbrain development
enteroendocrine cell differentiation
positive regulation of cell differentiation
positive regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of dendrite morphogenesis
transdifferentiation
axon development
transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
positive regulation of sequence-specific DNA binding transcription factor activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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