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Invitrogen
ESRRB Recombinant Rabbit Monoclonal Antibody (9H8L3)
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FIGURE: 1 / 2
ESRRB Antibody (703237) in ICC/IF
For immunofluorescence analysis, iPS cells were fixed and permeabilized for detection of endogenous ESRRB using Anti-ESRRB Recombinant Rabbit Monoclonal Antibody (Product # 703237, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) Panel a-d) shows re... View More
Product Details
703237
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
9H8L3
Immunogen
Peptide corresponding to human ESRRB [aa73-aa86]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Horse, Pig, Cat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
ESRRB (steroid hormone receptor ERR2) plays a role in maintenance of self-renewal and pluripotency of embryonic and trophoblast stem cells through different signaling pathways including FGF signaling pathway and Wnt signaling pathways. Upon FGF signaling pathway activation ESRRB interacts with KDM1A by directly binding to enhancer sites of ELF5 and EOMES and activates their transcription leading to self-renewal of trophoblast stem cells. ESSRB also regulates expression of multiple rod-specific genes and is required for survival of this cell type. Defects in the gene can cause deafness, autosomal recessive, 35 (DFNB35).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ERR beta-2; ERR-beta; ERRbeta; Estrogen receptor-like 2; estrogen-related nuclear receptor beta; Estrogen-related receptor beta; nuclear receptor ERRB2; Nuclear receptor subfamily 3 group B member 2; orphan nuclear receptor; Steroid hormone receptor ERR2; unnamed protein product
Gene Aliases: DFNB35; ERR beta-2; ERR2; ERRb; ERRB2; ERRbeta2; ESRL2; ESRRB; NR3B2
UniProt ID: (Human) O95718
Entrez Gene ID: (Human) 2103
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
RNA polymerase II core binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
steroid binding
protein binding
zinc ion binding
estrogen response element binding
sequence-specific DNA binding
metal ion binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
sequence-specific double-stranded DNA binding
embryonic placenta development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cell proliferation
stem cell division
stem cell population maintenance
intracellular steroid hormone receptor signaling pathway
intracellular receptor signaling pathway
somatic stem cell population maintenance
cell dedifferentiation
photoreceptor cell maintenance
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
inner ear development
morula formation
positive regulation of stem cell population maintenance
regulation of stem cell division
negative regulation of stem cell differentiation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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