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Invitrogen
LATS2 Recombinant Rabbit Monoclonal Antibody (17H14L2)
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FIGURE: 1 / 2
LATS2 Antibody (703621) in ICC/IF
For immunofluorescence analysis, U-2 OS cells were fixed and permeabilized for detection of endogenous LATS2 using Anti-LATS2 Recombinant Rabbit Monoclonal Antibody (Product # 703621, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) Panel a-d) shows ... View More
Product Details
703621
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
17H14L2
Immunogen
Protein corresponding to human LATS2 [aa500-aa648]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Horse, Rat, Mouse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
LATS2 is a serine/threonine protein kinase belonging to the LATS tumor suppressor family. LATS2 interacts with a negative regulator of p53 and function in a positive feedback loop with p53 that responds to cytoskeleton damage and this interaction provokes centrosome/mitotic apparatus dysfunction. LATS2 plays an essential role in the maintenance of mitotic fidelity and genomic integrity. LATS2 plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. It negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: h-warts; Kinase phosphorylated during mitosis protein; Large tumor suppressor homolog 2; LATS (large tumor suppressor, Drosophila) homolog 2; LATS, large tumor suppressor, homolog 2; serine/threonine kinase KPM; Serine/threonine-protein kinase kpm; Serine/threonine-protein kinase LATS2; unnamed protein product; Warts-like kinase
Gene Aliases: KPM; LATS2
UniProt ID: (Human) Q9NRM7
Entrez Gene ID: (Human) 26524
G1/S transition of mitotic cell cycle
inner cell mass cell fate commitment
inner cell mass cellular morphogenesis
protein phosphorylation
protein localization
hormone-mediated signaling pathway
regulation of transforming growth factor beta receptor signaling pathway
keratinocyte differentiation
hippo signaling
positive regulation of hippo signaling
intracellular signal transduction
positive regulation of apoptotic process
mitotic cell cycle phase transition
negative regulation of cyclin-dependent protein serine/threonine kinase activity
regulation of organ growth
cell division
canonical Wnt signaling pathway
negative regulation of canonical Wnt signaling pathway
negative regulation of protein localization to nucleus
positive regulation of NLRP3 inflammasome complex assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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