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FIGURE: 1 / 2
INSR beta Antibody (710076) in WB
Western blot analysis of IR beta in whole cell extracts of HeLa cells treated with 100 ng/mL insulin for 15 min (lane1); no treatment (lane 2); or treated (150 ng/mL IGF1 after serum starvation (lane 3) using an IR beta Recombinant Rabbit Superclonal™ Antibody (Product # 710076) at a dilution of 2.5 µg/mL.
Product Details
710076
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
6HCLC
Immunogen
Recombinant protein corresponding to amino acids 1283-1382 of human IR beta subunit
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
Biological actions of insulin and IGF-1 are mediated by their respective cell surface receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades. The insulin receptor. Insulin/IGF-1 binding to the extracellular domain leads to autophosphorylation of downstream target proteins. These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains, resulting in significant differences in the functional consequences of activation of each receptor. Defects in IR are the cause of various insulin resistance syndromes and IGF-1R defects may cause some forms of growth retardation. Both these signaling cascades are also important for the development of cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD220; CD220 beta; CD221; IGF-I receptor; Insulin receptor; insulin receptor precursor; IR; IR beta; unnamed protein product
Gene Aliases: CD220; HHF5; INSR
UniProt ID: (Human) P06213
Entrez Gene ID: (Human) 3643
nucleotide binding
beta-amyloid binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
insulin-activated receptor activity
insulin-like growth factor receptor binding
structural molecule activity
protein binding
ATP binding
GTP binding
kinase activity
transferase activity
protein domain specific binding
protein kinase activator activity
insulin-like growth factor I binding
insulin-like growth factor II binding
cargo receptor activity
identical protein binding
phosphatidylinositol 3-kinase binding
insulin binding
insulin receptor substrate binding
macromolecular complex binding
PTB domain binding
positive regulation of receptor internalization
heart morphogenesis
regulation of glycogen biosynthetic process
regulation of transcription, DNA-templated
receptor-mediated endocytosis
transmembrane receptor protein tyrosine kinase signaling pathway
G-protein coupled receptor signaling pathway
learning
memory
positive regulation of cell proliferation
insulin receptor signaling pathway
epidermis development
male gonad development
organ morphogenesis
regulation of gene expression
positive regulation of macromolecule biosynthetic process
regulation of glucose metabolic process
male sex determination
adrenal gland development
positive regulation of cell migration
exocrine pancreas development
receptor internalization
cellular response to insulin stimulus
glucose homeostasis
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of protein complex disassembly
positive regulation of MAPK cascade
positive regulation of nitric oxide biosynthetic process
positive regulation of glycogen biosynthetic process
positive regulation of glycolytic process
positive regulation of mitotic nuclear division
positive regulation of transcription, DNA-templated
regulation of embryonic development
positive regulation of glucose import
viral entry into host cell
protein autophosphorylation
animal organ development
positive regulation of developmental growth
system development
positive regulation of cellular component organization
regulation of multicellular organismal process
positive regulation of meiotic cell cycle
positive regulation of protein kinase B signaling
positive regulation of respiratory burst
cellular response to growth factor stimulus
dendritic spine maintenance
beta-amyloid clearance
transport across blood-brain barrier
neuron projection maintenance
regulation of female gonad development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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