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FIGURE: 1 / 6
IkB alpha Antibody (710128) in IHC (P)
Immunohistochemistry analysis of I-kappa-B-alpha showing staining in the cytoplasm of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room tempe... View More
Product Details
710128
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
6HCLC
Immunogen
Recombinant protein corresponding to amino acids 1-110 of human NF-kappa-B inhibitor alpha
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
IkB-alpha is a 40 kDa protein that functions to inhibit NF- kappaB activity. The inhibition occurs via protein-protein interaction between I kappaB proteins and NF- kappaB dimers in the cytosol. The interaction of I kappa B-alpha with NF- kappaB masks the nuclear localization sequence of NF- kappaB, preventing NF- kappaB translocation to the nucleus. A variety of stimuli can activate gene expression by liberating NF- kappaB through the degradation of I kappaB alpha. These stimuli include the proinflammatory cytokines TNF- alpha and IL-1 beta, chemokines, PMA, growth factors, LPS, UV irradiation, viral infection, as well as various chemical and physical stresses. In humans, the gene is located on the q arm of chromosome 14. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase alpha (IKK alpha), previously designated CHUK, interacts with IkB-alpha and specifically phosphorylates IkB-alpha on the sites that trigger its degradation Serines 32 and 36. IKK alpha appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKK alpha is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKK alpha, IKK beta and IKK gamma, and each appear to make essential contributions to IkB phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: I(Kappa)B(alpha); I-kappa-B-alpha; I-kappaBalpha; IkappaBalpha; IkB-alpha; IkBa; IKBalpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells alpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells, alpha; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; Mutations: 945:OK; NF-kappa-B inhibitor alpha; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha; nuclear factor of kappa light polyp gene enhancer in B-cell 1; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; RL/IF-1; unnamed protein product
Gene Aliases: AI462015; EDAID2; IKBA; MAD-3; MAD3; NFKBI; NFKBIA; RL/IF-1
UniProt ID: (Human) P25963, (Rat) Q63746, (Mouse) Q9Z1E3
Entrez Gene ID: (Human) 4792, (Rat) 25493, (Mouse) 18035
protein import into nucleus, translocation
cytoplasmic sequestering of NF-kappaB
regulation of gene expression
negative regulation of macrophage derived foam cell differentiation
positive regulation of cholesterol efflux
negative regulation of lipid storage
lipopolysaccharide-mediated signaling pathway
negative regulation of NF-kappaB transcription factor activity
positive regulation of cellular protein metabolic process
response to muramyl dipeptide
response to lipopolysaccharide
toll-like receptor 4 signaling pathway
response to muscle stretch
regulation of cell proliferation
cytoplasmic sequestering of transcription factor
response to exogenous dsRNA
negative regulation of myeloid cell differentiation
negative regulation of Notch signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
nucleotide-binding oligomerization domain containing 1 signaling pathway
nucleotide-binding oligomerization domain containing 2 signaling pathway
negative regulation of transcription from RNA polymerase II promoter
immune system process
regulation of transcription from RNA polymerase II promoter
protein import into nucleus
inflammatory response
Notch signaling pathway
I-kappaB kinase/NF-kappaB signaling
signal transduction involved in regulation of gene expression
negative regulation of cholesterol transport
tumor necrosis factor-mediated signaling pathway
NIK/NF-kappaB signaling
negative regulation of protein import into nucleus
negative regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of inflammatory response
B cell receptor signaling pathway
positive regulation of transcription initiation from RNA polymerase II promoter
cellular response to cold
interleukin-1-mediated signaling pathway
cellular response to cytokine stimulus
cellular response to tumor necrosis factor
negative regulation of cytokine production involved in inflammatory response
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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