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STAT6 Antibody (710140) in WB
Western blot analysis of STAT6 was performed by loading 20 µg of HeLa (lane1), A431 (lane2) and A549 (lane3) cell lysates using Novex®NuPAGE®4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Proteins were transferred to a PVDF membrane and blocked with 5 % skim milk for 1 hour at room temperature. STAT6 was detected at ~100 kDa using STAT6 Recombinant Rabbit Superclonal™ Antibody (Product # 710140) at 0.5 µg-1 µg/mL in 2.5 % ski... View More
Product Details
710140
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
21HCLC
Immunogen
Peptide corresponding to amino acids 630-638 of human STAT6
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
STAT6 protein is a transcription factor activated by cytokines, particularly interleukin-4 and IL13. (Stat6-/-) were found to be deficient in IL-4-mediated functions including Th2 helper T-cell differentiation, expression of cell surface markers, T-cell proliferation, immunoglobulin class switching to IgE, and partial loss of IL-4 mediated proliferation. STAT6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung and placenta. STAT6 is critically involved in Th2 immune response. STAT6 is crucial for IL-4-mediated biological responses, and naturally in two isoforms: STAT6a and STAT6c.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 12S1644; IL-4 Stat; IL4 STAT; LITAF binding protein; Mutations: 16:OK; Signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT; STAT, interleukin4-induced; STAT-6; transcription factor IL-4 STAT; unnamed protein product
Gene Aliases: D12S1644; HIES6; IL-4-STAT; STAT6; STAT6B; STAT6C
UniProt ID: (Human) P42226
Entrez Gene ID: (Human) 6778
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription coactivator binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein phosphatase binding
identical protein binding
sequence-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
T-helper 1 cell lineage commitment
negative regulation of type 2 immune response
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
defense response
signal transduction
JAK-STAT cascade
gene expression
cytokine-mediated signaling pathway
mammary gland epithelial cell proliferation
interleukin-4-mediated signaling pathway
regulation of cell proliferation
response to peptide hormone
positive regulation of transcription from RNA polymerase II promoter
isotype switching to IgE isotypes
positive regulation of isotype switching to IgE isotypes
JAK-STAT cascade involved in growth hormone signaling pathway
mammary gland morphogenesis
regulation of mast cell proliferation
positive regulation of cold-induced thermogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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