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LMX1A Antibody (710980) in WB
Western blot analysis was performed on tissue extracts (30 µg lysate) of Rat Brain (Lane 1), Mouse Brain (Lane 2), Mouse Cerebellum (Lane 3) and Mouse heart (Lane 4). The blots were probed with Anti-Lmx1a Recombinant Rabbit Superclonal™ Antibody (Product # 710980, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 42 kDa band corresponding to Lmx1a was observed according to the tested tissues. Known quantity o... View More
Product Details
710980
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
20HCLC
Immunogen
Protein corresponding to Human LMX1A (aa 195-370)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Cat and Rabbit.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
The LIM homeobox transcription factor 1 (LMX1A) belongs to the LIM-homeodomain family. Members of this family are known to be important for pattern formation during development. LMX1A binds an A/T-rich sequence and functions in the nucleus as a transcriptional activator to the insulin gene promoter. In the developing embryo, LMX1A is expressed along the neuraxis and leads to the development of the roof plate of the vertebrae. While LMX1A is crucial for the differentiation of dopamine neurons, the related LMX1B appears to be important for both the development and the survival of these cells. It has been suggested that LMX1A might be a useful biomarker or risk factor in tumor aggressiveness.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: LIM homeobox transcription factor 1-alpha; LIM/homeobox protein 1.1; LIM/homeobox protein LMX1A; LMX-1; LMX-1.1; MGC87616; shaker short-tail; unnamed protein product
Gene Aliases: DFNA7; dr; dreher; LMX1; LMX1.1; LMX1A; sst
UniProt ID: (Human) Q8TE12, (Mouse) Q9JKU8
Entrez Gene ID: (Human) 4009, (Rat) 289201, (Mouse) 110648
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
protein binding
metal ion binding
sequence-specific double-stranded DNA binding
molecular_function
zinc ion binding
sequence-specific DNA binding
regulation of cell growth
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
axon guidance
central nervous system development
memory
locomotory behavior
regulation of gene expression
dentate gyrus development
cerebellum development
hippocampus development
central nervous system neuron differentiation
neuron differentiation
midbrain development
olfactory behavior
negative regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
synapse organization
dopaminergic neuron differentiation
midbrain dopaminergic neuron differentiation
brain development
transcription, DNA-templated
multicellular organism development
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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