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FIGURE: 1 / 2
MAPKAP1 Antibody (711539) in ICC/IF
For immunofluorescence analysis, MCF7 cells were fixed and permeabilized for detection of endogenous Sin1 using Anti- Sin1 Recombinant Rabbit Superclonal™ Antibody (Product # 711539, 2µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Sin1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
711539
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Immunogen
Protein corresponding to human MAPKAP1 (aa372-aa493)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Cat, Sheep, and Bovine.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
Sin1 was initially identified as the human homolog of S. pombe SIN1. Recent evidence has shown that it identical to Mip1, a protein that interacts with MEKK2, a member of the mitogen-activated protein kinase (MAPK) intracellular signaling network. MAPKAP1 is thought to prevent MEKK2 activation and dimerization by forming a complex with the inactive and non-phosphorylated MEKK2, thereby blocking the JNK1/2, ERK1/2, p38 and ERK5 MAPKs. MAPKAP1 has also been shown to play a role in the TOR signaling process, a pathway that is involved in controlling cell growth and proliferation in response to environmental cues such as nutrients, growth factors and hormones. Experiments showed that MAPKAP1 helped to maintain the TOR/rictor assembly but not the TOR/RAPTOR complex, which allowed specific phosphorylation of Akt, a kinase that is believed to couple the growth factor-PI3K signaling pathway to the nutrient-regulated TOR signaling pathway. Multiple alternatively spliced isoforms of MAPKAP1 have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MEKK2-interacting protein 1; MGC2745; Mip1a; Mip1b; Mip1r; MIP1SIN1SIN1b; mitogen-activated protein kinase 2-associated protein 1; mitogen-activated protein kinase associated protein 1; mitogen-activated protein kinase-associated protein 1; MAPKAP1; mSIN1; ras inhibitor MGC2745; RP11-269P11.1; SAPK-interacting protein 1; SIN1; SIN1a; SIN1b; SIN1g; stress-activated map kinase interacting protein 1; stress-activated map kinase-interacting protein 1; stress-activated protein kinase-interacting 1; target of rapamycin complex 2 subunit MAPKAP1-like; TORC2 subunit MAPKAP1; unnamed protein product
Gene Aliases: D230039K05Rik; JC310; MIP1; SIN1; SIN1b; SIN1g
UniProt ID: (Human) Q9BPZ7, (Mouse) Q8BKH7, (Rat) Q6AYF1
Entrez Gene ID: (Human) 79109, (Mouse) 227743, (Rat) 296648
protein serine/threonine kinase activity
protein binding
phosphatidylinositol-4,5-bisphosphate binding
phosphatidylinositol-3,4,5-trisphosphate binding
protein kinase binding
small GTPase binding
phosphatidylinositol-3,4-bisphosphate binding
binding, bridging
phosphatidic acid binding
phosphatidylinositol-3,5-bisphosphate binding
enzyme-substrate adaptor activity
ubiquitin-dependent protein catabolic process
cytoskeleton organization
insulin receptor signaling pathway
positive regulation of cell growth
cellular response to nutrient levels
cellular response to insulin stimulus
positive regulation of peptidyl-serine phosphorylation
TORC2 signaling
negative regulation of apoptotic process
protein kinase B signaling
negative regulation of Ras protein signal transduction
negative regulation of insulin receptor signaling pathway
positive regulation of protein kinase B signaling
regulation of cellular response to oxidative stress
substantia nigra development
biological_process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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