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FIGURE: 1 / 9
Ataxin 3 Antibody (711823) in ICC/IF
Knockdown of Ataxin 3 was achieved by transfecting PC-3 cells with specific siRNA (Silencer® select Product # s230538 & s8794). Immunofluorescence analysis was performed on PC-3 cells (untransfected, panel a-d), transfected with Ataxin 3 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labelled with Anti-Ataxin 3 Recombinant Rabbit Superclonal™ Antibody (Product # 711823, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor®... View More
Product Details
711823
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
13HCLC
Immunogen
Protein corresponding to human Ataxin-3 (Oligo) (aa1-aa364)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Horse, Dog
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
Machado-Joseph disease is an autosomal dominant neurologic disorder, and is now known to be the same as previously described spinocerebellar ataxia-3. MJD protein (Ataxin-3) contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. This protein interacts with key regulators (CBP, p300 and PCAF) of transcription and represses transcription, and also acts as a histone-binding protein that regulates transcription. MJD is a deubiquitinating enzyme.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Ataxin-3; ataxin3; josephin; Machado-Joseph disease protein 1; olivopontocerebellar ataxia 3; spin; Spinocerebellar ataxia type 3 protein
Gene Aliases: AT3; ATX3; ATXN3; JOS; MJD; MJD1; SCA3
UniProt ID: (Human) P54252
Entrez Gene ID: (Human) 4287
microtubule cytoskeleton organization
cytoplasmic translation
nucleotide-excision repair
proteolysis
ubiquitin-dependent protein catabolic process
misfolded or incompletely synthesized protein catabolic process
chemical synaptic transmission
nervous system development
cellular response to starvation
regulation of cell-substrate adhesion
protein deubiquitination
actin cytoskeleton organization
cellular response to nutrient levels
cellular response to amino acid starvation
cellular response to heat
monoubiquitinated protein deubiquitination
exploration behavior
TORC1 signaling
proteasome-mediated ubiquitin-dependent protein catabolic process
intermediate filament cytoskeleton organization
negative regulation of translational initiation
positive regulation of translational initiation
protein K63-linked deubiquitination
protein K48-linked deubiquitination
cellular response to misfolded protein
negative regulation of TORC1 signaling
positive regulation of TORC1 signaling
positive regulation of ERAD pathway
protein localization to cytosolic proteasome complex
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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