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FIGURE: 1 / 6
IRF3 Antibody (712217) in ICC/IF
For immunofluorescence analysis, A549 cells were fixed and permeabilized for detection of endogenous IRF3 using Anti-IRF3 Recombinant Rabbit Superclonal™ Antibody (Product # 712217, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) Panel a-d) shows re... View More
Product Details
712217
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
1HCLC
Immunogen
Protein corresponding to human IRF3 [aa1-aa427]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Cat, Bat.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
IFR3 (interferon regulatory factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses. It plays a critical role in the innate immune response against DNA and RNA viruses. IFR3 regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. It acts as a more potent activator of the IFN-beta gene than the IFN-alpha gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. IFR3 is found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA, or toll-like receptor signaling. It is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Mutations in the gene can result in encephalopathy, acute, infection-induced, herpes-specific 7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Interferon regulatory factor 3; IRF-3; IRF-3a; IRF-3b; IRF-3c; IRF-3d; IRF-3e; IRF-3f; IRF3 (phospho S386); IRF3 (pS386); IRF3 (pSer386); IRF3 phospho; IRF3 phospho S386; IRF3 phospho Ser386; phospho IRF3; unnamed protein product
Gene Aliases: IIAE7; IRF3
UniProt ID: (Human) Q14653
Entrez Gene ID: (Human) 3661
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein domain specific binding
identical protein binding
protein homodimerization activity
sequence-specific DNA binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
negative regulation of transcription from RNA polymerase II promoter
positive regulation of cytokine production
immune system process
cytoplasmic pattern recognition receptor signaling pathway
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
apoptotic process
cellular response to DNA damage stimulus
cell surface receptor signaling pathway
mRNA transcription
response to bacterium
regulation of gene expression
programmed cell death
signal transduction involved in regulation of gene expression
lipopolysaccharide-mediated signaling pathway
positive regulation of type I interferon production
response to lipopolysaccharide
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
toll-like receptor 4 signaling pathway
TRIF-dependent toll-like receptor signaling pathway
MDA-5 signaling pathway
regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to exogenous dsRNA
innate immune response
positive regulation of innate immune response
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of inflammatory response
defense response to virus
type I interferon signaling pathway
positive regulation of type I interferon-mediated signaling pathway
cellular response to lipopolysaccharide
cellular response to exogenous dsRNA
macrophage apoptotic process
programmed necrotic cell death
cellular response to virus
antiviral innate immune response
cGAS/STING signaling pathway
positive regulation of cytokine production involved in inflammatory response
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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