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NeoBiotechnologies
Topoisomerase II alpha Recombinant Mouse Monoclonal Antibody (rTOP2A, 6569)
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FIGURE: 1 / 5
Topoisomerase II alpha Antibody (7153-MSM4-P1ABX) in IHC (P)
Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human brain using Topoisomerase II alpha Recombinant Monoclonal Antibody (Product # 7153-MSM4-P1ABX) at 2 µg/mL in PBS for 30 min RT. HIER: Tris/EDTA, pH9.0, 45 min. Secondary: HRP-polymer, 30 min. DAB, 5 min.
Product Details
7153-MSM4-P1ABX
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
rTOP2A, 6569
Immunogen
Recombinant human Topoisomerase II alpha fragment (aa1352-1493)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Target Information
Topo II alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2 and M-phases in transformed and developmentally regulated normal cellsTopo II alpha is also implicated in drug resistance of tumor cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA gyrase; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase 2-alpha; DNA topoisomerase II (EC 5.99.1.3); DNA Topoisomerase II alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; EC 5.99.1.3; OTTHUMP00000207108; P11388-1; TOP2a/90; topoisomerase (DNA) II alpha 170kDa; topoisomerase ii alpha
Gene Aliases: Top-2; TOP2; TOP2A; TOP2alpha; TOPIIA; TP2A
UniProt ID: (Human) P11388, (Mouse) Q01320
Entrez Gene ID: (Human) 7153, (Mouse) 21973
nucleotide binding
magnesium ion binding
DNA binding
chromatin binding
RNA binding
DNA topoisomerase activity
DNA topoisomerase type II (ATP-hydrolyzing) activity
protein kinase C binding
protein binding
ATP binding
DNA-dependent ATPase activity
DNA binding, bending
isomerase activity
protein homodimerization activity
ubiquitin binding
metal ion binding
protein heterodimerization activity
protein C-terminus binding
drug binding
enzyme binding
histone deacetylase binding
sequence-specific DNA binding
structure-specific DNA binding
poly(A) RNA binding
DNA metabolism protein
resolution of meiotic recombination intermediates
sister chromatid segregation
hematopoietic progenitor cell differentiation
DNA metabolic process
DNA topological change
chromatin organization
cellular response to DNA damage stimulus
chromosome segregation
female meiotic division
chromosome condensation
apoptotic chromosome condensation
embryonic cleavage
regulation of circadian rhythm
positive regulation of apoptotic process
positive regulation of single stranded viral RNA replication via double stranded DNA intermediate
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
DNA ligation
DNA unwinding involved in DNA replication
mitotic DNA integrity checkpoint
positive regulation of viral genome replication
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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