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Invitrogen

GATA-3 Recombinant Rat Monoclonal Antibody (TWAJ)

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Product Details

740023T

Applications
Tested Dilution
Publications

Western Blot (WB)

1:400
-

Immunocytochemistry (ICC/IF)

1:200
-

Flow Cytometry (Flow)

0.016-0.25 µg/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rat / IgG2b, kappa

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

TWAJ

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2-7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3666212

Product Specific Information

Recombinant rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Target Information

The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: GATA binding protein-3; GATA-3; GATA-binding factor 3; MGC2346; MGC5199; MGC5445; Transacting T-cell-specific transcription factor GATA-3; unnamed protein product

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Gene Aliases: HDR; HDRS

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UniProt ID: (Human) P23771

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Entrez Gene ID: (Human) 2625

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcription coactivator binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding interleukin-2 receptor binding protein binding zinc ion binding identical protein binding sequence-specific DNA binding RNA polymerase II sequence-specific DNA binding transcription factor binding E-box binding HMG box domain binding histone methyltransferase binding sequence-specific double-stranded DNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter cell fate determination type IV hypersensitivity regulation of cytokine production kidney development mesonephros development immune system development aortic valve morphogenesis cardiac right ventricle morphogenesis ventricular septum development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter defense response inflammatory response signal transduction negative regulation of cell proliferation male gonad development response to xenobiotic stimulus response to virus anatomical structure morphogenesis positive regulation of signal transduction response to gamma radiation gene expression positive regulation of endothelial cell migration negative regulation of epithelial to mesenchymal transition cell differentiation T cell differentiation macrophage differentiation regulation of epithelial cell differentiation TOR signaling positive regulation of interleukin-13 production positive regulation of interleukin-4 production positive regulation of interleukin-5 production negative regulation of mammary gland epithelial cell proliferation cellular response to interferon-alpha norepinephrine biosynthetic process protein kinase B signaling ear development response to estrogen T-helper 2 cell differentiation cell fate commitment response to ethanol positive regulation of T cell differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter sympathetic nervous system development embryonic organ development anatomical structure formation involved in morphogenesis system development negative regulation of inflammatory response regulation of developmental process T cell receptor signaling pathway cartilage development positive regulation of protein kinase B signaling pharyngeal system development uterus development canonical Wnt signaling pathway mesenchymal to epithelial transition ureteric bud formation cellular response to interleukin-4 cellular response to tumor necrosis factor positive regulation of ureteric bud formation nephric duct morphogenesis nephric duct formation regulation of nephron tubule epithelial cell differentiation lymphocyte migration cochlea development positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of cell motility negative regulation of endothelial cell apoptotic process positive regulation of thyroid hormone generation positive regulation of transcription regulatory region DNA binding regulation of cellular response to X-ray
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