Biodyne™ B Nylon Membrane, 0.45 µm, 8 cm x 12 cm

Catalog number: 77016

Thermo Scientific™  Related applications: Nucleic Acid Gel Electrophoresis & Blotting

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Thermo Scientific Biodyne B Nylon Membranes are sheets of positively charged nylon that provide excellent binding characteristics for nucleic acid transfer, blotting and detection methods.

Features of Biodyne B Nylon Membranes:

Versatile and durable—achieve strong DNA binding by baking or UV-crosslinking without damaging the membrane
Easy to use—pre-cut 8 x 12 cm sheets are the ideal size for standard mini-gel transfers and microplate dot-blots
High signal-to-noise ratio—lower background than nitrocellulose and most other nylon transfer membranes

Biodyne B Nylon Membrane is manufactured with a matrix pore size of 0.4 micron, and is supplied in packages of 8 x 12 cm sheets. Biodyne B Membrane contains exclusively positive charges, providing the highest possible binding capacity for negatively charged molecules like DNA and RNA. The membrane is resistant to heat and solvents and will not shrink, crack or tear.

Properties of Biodyne B Nylon Membrane:
• Composition: positively charged Nylon 66
• Surface: strong cationic quaternary ammonium groups provide positive zeta potential, even to pH 10
• Properties: excellent for DNA, RNA and other negatively charged molecules
• Applications: Southern blotting, Northern blotting, DNA and RNA methods, including gel shift assays with Thermo Scientific LightShift Chemiluminescent EMSA Kits

More Product Data
Transfer EMSA gels using the Pierce G2 Fast Blotter

Related Products
Biodyne™ A Nylon Membrane, 0.45 µm, 8 cm x 12 cm
For Research Use Only. Not for use in diagnostic procedures.


Membrane: Nylon Membrane
Pore Size: 0.45 micron
Product Size: 25 sheets

Contents & storage

Note: Always wear gloves when handling membranes because oils from fingers may prevent proper wetting. Also, proteins and other contaminants from hands may bind to the membranes and cause background.


Manuals & protocols

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