Thermo Scientific B-PER Reagent (in Phosphate Buffer) is an easy-to-use cell lysis reagent is a nonionic detergent-based solutions that effectively disrupt cells and solubilize native or recombinant proteins without denaturation. All B-PER Reagents are compatible with downstream applications, such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay). Depending on the particular application, protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.
Features of the B-PER Bacterial Protein Extraction Reagent:
• Detergent in sodium phosphate buffer; no enzyme components • Amine-free formulation for direct compatibility of lysate with amine-reactive labeling and crosslinking
All Thermo Scientific B-PER Protein Extraction Reagents are: • Ready to use—one-step cell lysis of gram-positive and gram-negative bacteria using a mild, nonionic detergent (proprietary) in Tris or phosphate buffer formulations • Fast and simple—just add B-PER Reagent to a bacterial pellet, incubate with mixing for 10 to 15 minutes and recover soluble proteins after pelleting the cell debris • Convenient – B-PER Complete Reagent contains lysozyme and a universal nuclease in a single formulation with 4°C storage • Excellent yields—recover recombinant proteins from bacterial lysates or purify inclusion bodies to near-homogeneous levels • Flexible—B-PER Reagents are suitable for any scale of protein extraction and are available in phosphate and 1X and 2X Tris formulations, with and without enzymes • Compatible—completely compatible with addition of protease inhibitors, and the resulting protein extract can be used in protein assays, typical affinity purification methods (e.g., GST, 6xHis) and other applications
Convenient, Ready-to-use Formats B-PER Bacterial Extraction Reagents are more effective than traditional sonication and typical homemade lysis buffers, many of which include detergents and components that interfere with downstream applications. B-PER Reagents are formulated in Tris or phosphate buffer at physiological pH. They extract native and soluble recombinant proteins and yield lysates that are directly compatible with most downstream workflows such as electrophoresis, affinity purification, immunoprecipitation, protein interaction analysis, crosslinking and protein labeling.