Cóctel inhibidor de proteasas y fosfatasas Halt™ (100X)
Cóctel inhibidor de proteasas y fosfatasas Halt™ (100X)
Citas y referencias (7)
Thermo Scientific™

Cóctel inhibidor de proteasas y fosfatasas Halt™ (100X)

El cóctel inhibidor de proteasas y fosfatasas Thermo Scientific Halt (100X) proporciona la comodidad de una solución única con laMás información
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Número de catálogoCantidad
7844610 ml
7844224 × 100 μl
784401 ml
784445 x 1 ml
Número de catálogo 78446
Precio (MXN)
-
Cantidad:
10 ml
Pedido a granel o personalizado
El cóctel inhibidor de proteasas y fosfatasas Thermo Scientific Halt (100X) proporciona la comodidad de una solución única con la protección completa de las muestras de proteínas para lisados de células y tejidos.

Características del cóctel inhibidor de proteasas y fosfatasas Halt (100X):

• Protección completa: cóctel todo en uno que contiene inhibidores de proteasas y fosfatasas.
• Envases de varios tamaños: seleccione uno de los cuatro prácticos tamaños de envase que se ajustan a prácticamente todas las necesidades.
• Práctico: la formulación del líquido 100X es mucho más sencilla y eficaz que los inhibidores individuales y otros formatos.
• Fácil almacenamiento: el formato con base acuosa permite un práctico almacenamiento en sala fría sin congelación, a diferencia de los cócteles basados en DMSO
• Compatible: se puede usar en espectrometría de masas (MS) porque no contiene AEBSF, lo cual puede provocar que los picos se desplacen.
• Validado: totalmente compatible con los tampones de lisis celular Thermo Scientific Pierce.

El cóctel de inhibidores de proteasas y fosfatasas Halt está específicamente optimizado para proteger las proteínas de la degradación durante la extracción y purificación. El cóctel contiene inhibidores contra las principales clases de proteasas y fosfatasas, por lo que se ocupa de aminopeptidasas, proteasas de cisteína y serina, y fosfatasas de serina/treonina y proteína tirosina. Para promover la inhibición de las metaloproteasas, el ácido etildiaminotetraacético (EDTA) a 0,5 M se suministra en un tubo independiente.

Productos relacionados
Cóctel inhibidor de proteasa y fosfatasa Halt™, sin EDTA (100X)

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
TampónTampón de lisis
InhibidoresInhibidor de la proteasa, inhibidor de la fosfatasa
Cantidad10 ml
Tipo de reactivoInhibidor de la proteasa, inhibidor de la fosfatasa
FormularioLíquido
Línea de productosHalt
Tipo de productoCóctel inhibidor
Unit SizeEach
Contenido y almacenamiento
Tras su recepción, almacenar a 4 °C

Preguntas frecuentes

Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?

The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?

Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?

EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?

The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Cóctel inhibidor de proteasas y fosfatasas Halt™ (100X) FAQs

Citations & References (7)

Citations & References
Abstract
A guide to selecting high-performing antibodies for human Midkine for use in Western blot and immunoprecipitation.
Authors:Ayoubi R,Southern K,Laflamme C,NeuroSGC/YCharOS Collaborative Group
Journal:F1000Research
PubMed ID:39092005
Midkine is a secreted protein that acts as a growth factor or cytokine involved in cell survival and inflammatory processes. It accumulates in amyloid plaques, which are hallmarks of Alzheimer's Disease (AD). The reproducibility of Midkine research would be enhanced if the community had access to well-characterized anti-Midkine antibodies. In ... More
Chemoproteomic discovery of a covalent allosteric inhibitor of WRN helicase.
Authors:Baltgalvis KA,Lamb KN,Symons KT,Wu CC,Hoffman MA,Snead AN,Song X,Glaza T,Kikuchi S,Green JC,Rogness DC,Lam B,Rodriguez-Aguirre ME,Woody DR,Eissler CL,Rodiles S,Negron SM,Bernard SM,Tran E,Pollock J,Tabatabaei A,Contreras V,Williams HN,Pastuszka MK,Sigler JJ,Pettazzoni P,Rudolph MG,Classen M,Brugger D,Claiborne C,Plancher JM,Cuartas I,Seoane J,Burgess LE,Abraham RT,Weinstein DS,Simon GM,Patricelli MP,Kinsella TM
Journal:Nature
PubMed ID:38658751
WRN helicase is a promising target for treatment of cancers with microsatellite instability (MSI) due to its essential role in resolving deleterious non-canonical DNA structures that accumulate in cells with faulty mismatch repair mechanisms(1-5). Currently there are no approved drugs directly targeting human DNA or RNA helicases, in part owing ... More
Selection of Leptin Surrogates by a General Phenotypic Screening Method for Receptor Agonists.
Authors:Wang T,Chen X,Yang G,Shi X
Journal:Biomolecules
PubMed ID:38672473
There is a high demand for agonist biomolecules such as cytokine surrogates in both biological and medicinal research fields. These are typically sourced through natural ligand engineering or affinity-based screening, followed by individual functional validation. However, efficient screening methods for identifying rare hits within immense libraries are very limited. In ... More
hCLCA1 and mCLCA3 are secreted non-integral membrane proteins and therefore are not ion channels.
Authors:Gibson A, Lewis AP, Affleck K, Aitken AJ, Meldrum E, Thompson N
Journal:J Biol Chem
PubMed ID:15919655
'Proteins of the CLCA gene family have been proposed to mediate calcium-activated chloride currents. In this study, we used detailed bioinformatics analysis and found that no transmembrane domains are predicted in hCLCA1 or mCLCA3 (Gob-5). Further analysis suggested that they are globular proteins containing domains that are likely to be ... More
Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.
Authors:Ståhlberg A, Thomsen C, Ruff D, Åman P
Journal:Clin Chem
PubMed ID:23014600
The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. ... More
View all Cóctel inhibidor de proteasas y fosfatasas Halt™ (100X) citations