A guide to selecting high-performing antibodies for human Midkine for use in Western blot and immunoprecipitation.
AuthorsAyoubi R,Southern K,Laflamme C,NeuroSGC/YCharOS Collaborative Group
JournalF1000Research
PubMed ID39092005
Midkine is a secreted protein that acts as a growth factor or cytokine involved in cell survival and inflammatory processes. It accumulates in amyloid plaques, which are hallmarks of Alzheimer's Disease (AD). The reproducibility of Midkine research would be enhanced if the community had access to well-characterized anti-Midkine antibodies. In ... More
Chemoproteomic discovery of a covalent allosteric inhibitor of WRN helicase.
WRN helicase is a promising target for treatment of cancers with microsatellite instability (MSI) due to its essential role in resolving deleterious non-canonical DNA structures that accumulate in cells with faulty mismatch repair mechanisms(1-5). Currently there are no approved drugs directly targeting human DNA or RNA helicases, in part owing ... More
Selection of Leptin Surrogates by a General Phenotypic Screening Method for Receptor Agonists.
AuthorsWang T,Chen X,Yang G,Shi X
JournalBiomolecules
PubMed ID38672473
There is a high demand for agonist biomolecules such as cytokine surrogates in both biological and medicinal research fields. These are typically sourced through natural ligand engineering or affinity-based screening, followed by individual functional validation. However, efficient screening methods for identifying rare hits within immense libraries are very limited. In ... More
hCLCA1 and mCLCA3 are secreted non-integral membrane proteins and therefore are not ion channels.
AuthorsGibson A, Lewis AP, Affleck K, Aitken AJ, Meldrum E, Thompson N
JournalJ Biol Chem
PubMed ID15919655
'Proteins of the CLCA gene family have been proposed to mediate calcium-activated chloride currents. In this study, we used detailed bioinformatics analysis and found that no transmembrane domains are predicted in hCLCA1 or mCLCA3 (Gob-5). Further analysis suggested that they are globular proteins containing domains that are likely to be ... More
Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.
AuthorsStåhlberg A, Thomsen C, Ruff D, Åman P
JournalClin Chem
PubMed ID23014600
The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. ... More
A small-molecule inhibitor targeting the mitotic spindle checkpoint impairs the growth of uterine leiomyosarcoma.
AuthorsShan W, Akinfenwa PY, Savannah KB, Kolomeyevskaya N, Laucirica R, Thomas DG, Odunsi K, Creighton CJ, Lev DC, Anderson ML
JournalClin Cancer Res
PubMed ID22535157
Uterine leiomyosarcoma (ULMS) is a poorly understood cancer with few effective treatments. This study explores the molecular events involved in ULMS with the goal of developing novel therapeutic strategies. ... More
The Src inhibitor dasatinib accelerates the differentiation of human bone marrow-derived mesenchymal stromal cells into osteoblasts.
AuthorsId Boufker H, Lagneaux L, Najar M, Piccart M, Ghanem G, Body JJ, Journé F
JournalBMC Cancer
PubMed ID20565769
The proto-oncogene Src is an important non-receptor protein tyrosine kinase involved in signaling pathways that control cell adhesion, growth, migration and differentiation. It negatively regulates osteoblast activity, and, as such, its inhibition is a potential means to prevent bone loss. Dasatinib is a new dual Src/Bcr-Abl tyrosine kinase inhibitor initially ... More