T-PER™ Tissue Protein Extraction Reagent - FAQs

The T-PER Tissue Protein Extraction Reagent (Cat. No. 78510) is compatible with standard protein assays such as Pierce BCA Protein Assay (Cat. No. 23225) and Coomassie Plus (Bradford) Assay Kit (Cat. No. 23236).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

T-PER Tissue Protein Extraction Reagent is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (room temperature). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

The T-PER Tissue Protein Extraction Reagent is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (room temperature). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

For extraction of protein from tissue samples as well as some difficult-to-lyse mammalian cells, we recommend using T-PER Tissue Protein Extraction Reagent (Cat. No. 78510) with homogenization.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Homogenize tissue sample in 1:20 (w/v) of tissue to T-PER Reagent, then centrifuge to pellet cell or tissue debris, and collect supernatant. For example, use a ratio of 100 mg of tissue to 2 mL T-PER Reagent. Use a smaller volume of reagent if a more concentrated protein extract is required.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

T-PER Tissue Protein Extraction Reagent is shipped at ambient temperature. We recommend storing the product at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

The prepared cell lysate may be used for immunoassays (Western blots, ELISAs, RIAs), reporter assays (luciferase, β-galactosidase, chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, tyrosine kinase), and protein purification.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Yes, the reagent can be added directly to tissue that has been prepared using a mortar and pestle or any other mechanical means.

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Fresh tissue is always the best though frozen tissue can also be used with the reagent. Paraffin embedded or preserved tissue may present a problem for the lysis reagent, since the reagent does not permeate the cells in the tissue uniformly, so protein yield will be reduced. Mechanical disruption of the tissue is still required for all types of tissue. A hand-held homogenizer may be used, or the tissue may be frozen in liquid nitrogen and then ground.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

The T-PER Tissue Protein Extraction Reagent is compatible with Thermo Scientific Coomassie Plus (Bradford) Assay (Cat. No. 23236). It is also compatible with Thermo Scientific BCA Protein Assay (Cat. No. 23225) and Thermo Scientific 660 nm Protein Assay (Cat. No. 22660) after a 1:2 dilution.

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No. Both phases will be extracted together. We recommend Mem-PER PLUS Membrane Protein Extraction Kit (Cat. No. 89842) and NE-PER Nuclear and Cytoplasmic Extraction Reagents (Cat. No. 78833) to isolate membrane and nuclear proteins, respectively from tissue samples

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Mouse heart, liver, kidney, and brain tissues have been tested with the T-PER Tissue Protein Extraction Reagent. This product performs exceptionally well for tumor growth factor and insulin growth factor extraction. T-PER Reagent was validated for protein yield in both fresh and frozen tissue. Additionally, T-PER Reagent was shown to efficiently extract target proteins from various cellular compartments such as plasma membrane (Na-K+ ATPase, pan-cadherin), cytoplasm (HSP90, MAPK), mitochondria (COXIV), and nucleus (HDAC1), and tissue specific markers from brain (synaptophysin) and heart tissue (Troponin T Cardiac Isoform).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

T-PER Tissue Protein Extraction Reagent is provided as a 500 mL volume, and contains a mild zwitterionic dialyzable detergent in 25 mM Bicine, 150 mM sodium chloride at pH 7.6. The sodium chloride in T-PER Reagent was added to the formulation to make the reagent more compatible with downstream assays normally carried out from tissue preparations. We recommend adding Halt Protease Inhibitor Cocktail (Cat. No. 87785) to the T-PER Reagent just before use.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

T-PER Tissue Protein Extraction Reagent enables mild extraction of total protein from tissue samples. T-PER Reagent was designed for use with all tissues, independent of mechanical extraction methods. The mild detergent enhances solubilization of proteins without compromising function, and the neutral pH and salt concentration of this reagent enable efficient extraction from cellular compartments yielding homogeneous lysates.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Please see the various lysis buffers we offer (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/cell-lysis-fractionation/cell-lysis-total-protein-extraction.html) for total protein extraction from mammalian cells.

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.