Informational:Inquire about OEM or Commercial Supply version of this product here.
Informational:New Protein Purification and Isolation Support Center—Find technical tips and troubleshooting recommendations from our scientists.
Thermo Scientific™

Subcellular Protein Fractionation Kit for Cultured Cells

Catalog number: 78840
Thermo Scientific™

Subcellular Protein Fractionation Kit for Cultured Cells

Catalog number: 78840
Catalog Number
78840
Unit Size
35 mL
Price (USD)
Price: 566.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
7884035 mL
Price: 566.00
Your Price:
-

Recurring order eligible

Save time by having your items shipped automatically.

Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations
Thermo Scientific Subcellular Protein Fractionation Kit for Cultured Cells enables segregation and enrichment of proteins from five different cellular compartments.

Features of Subcellular Protein Fractionation Kit for Cultured Cells:

Concise—extract functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions from mammalian cultured cells in less than 3 hours
Convenient—perform a simple procedure without using ultracentrifugation over gradients
Complete—obtain cytoplasmic, membrane, soluble nuclear, chromatin-bound and cytoskeletal protein fractions from a single kit
Cultured Cells—developed and optimized for use with cultured mammalian cells; for tissue samples use the Subcellular Fractionation Kit for Tissues
Compatible—use extracts for downstream applications such as protein assays, Western blotting, protein assays, electrophoretic mobility shift assays, reporter-gene and enzyme-activity assays.

Applications for subcellular fractionation:
• Extract and enrich proteins from different cellular compartments
• Isolate cytoplasmic, nuclear, membrane and cytoskeletal proteins from the same cells
• Profile cells for proteins from the cytoplasm, nucleus, membrane and cytoskeleton
• Study protein translocation

Fractionation of subcellular proteins enables protein localization assessment and protein enrichment from specific cellular compartments. Thermo Scientific Subcellular Protein Fractionation Kit includes a combination of reagents for stepwise lysis of cells into functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions in less than 3 hours. Extracts from each subcellular compartment generally have less than 15% contamination between fractions, which is sufficient purity for most experiments studying protein localization and redistribution.

The Subcellular Protein Fractionation Kit for Cultured Cells contains four extraction buffers, a stabilized nuclease and Thermo Scientific Halt Protease Inhibitor Cocktail. Each kit has enough reagents to fractionate 50 cell pellets of 20 µL each, equivalent to approximately 2g of cell paste. (For tissue samples, use our Subcellular Protein Fractionation Kit for Tissue.)

The first kit reagent, when added to a cell pellet, causes selective membrane permeabilization, releasing soluble cytoplasmic contents. The second reagent dissolves plasma, mitochondria and ER-golgi membranes but does not solubilize the nuclear membranes. After recovering intact nuclei by centrifugation, a third reagent yields the soluble nuclear extract. An additional nuclear extraction with micrococcal nuclease is performed to release chromatin-bound nuclear proteins. The recovered insoluble pellet is then extracted with the final reagent to isolate cytoskeletal proteins.

Related Products
Subcellular Protein Fractionation Kit for Tissues
Benchtop Centrifuges
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity
1 Kit
Sufficient For
50 Samples
Buffer
Lysis Buffer
Form
Liquid
Reagent Type
Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation
Product Type
Subcellular Protein Fractionation Kit

Contents & Storage

Upon receipt store kit at -20°C, or store individual components as indicated above. Kit is shipped with dry ice.