Thermo Scientific™

Y-PER™ Yeast Protein Extraction Reagent

Catalog number: 78991
Thermo Scientific™

Y-PER™ Yeast Protein Extraction Reagent

Catalog number: 78991
Catalog Number
78991
Unit Size
200 mL
Price (USD)
186.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
500 mL
Price (USD)
397.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
78991200 mL
186.00
Your Price:
***
78990500 mL
397.00
Your Price:
***
Product Overview
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Thermo Scientific Y-PER Yeast Protein Extraction Reagent is the first commercially available yeast lysis reagent using a mild detergent lysis procedure to efficiently release functionally active solubilized proteins.

Features of Y-PER Yeast Protein Extraction Reagent:

More protein—extracts more than twice as much protein as glass bead methods
Less hassle—eliminates the difficulties associated with traditional glass bead lysis (e.g., clinging static-charged beads, protein-bead clumps and runaway beads)
Optmized for yeast—validated for use with Saccharomyces cerevisiae, Schizosaccharomyces pombe and Pichia pastoris
Versatile—effective for many different organisms, including Bacillus subtilus and both Gram-positive and Gram-negative bacteria; suitable for use in a diverse range of situations

The detergent-based cell lysis buffer eliminates the need to use glass beads or mechanical treatments involving strong reducing agents, chemicals, pH and temperature extremes to disrupt the proteinaceous cell envelope required for protein extractions. The popular glass bead lysis protocol requires special equipment and must be performed at 4°C. The low yields of protein commonly obtained with this technique are the result of denaturation and proteins nonspecifically binding to the glass beads. However, Y-PER Reagent uses a simple room temperature protocol that can be completed in 20 minutes and requires no special equipment.

Y-PER Reagent offers an easy to use standard protocol for protein extractions involving addition of an appropriate volume of Y-PER Reagent to pelleted yeast cells, followed by incubation at room temperature for approximately 20 minutes and then spin down the debris. The resulting supernatant is a concentrated protein solution, surpassing typical yields obtained with traditional glass bead disruption.

The unicellular nature of yeast, combined with its ability to perform post-translational modifications that closely mimic processes in higher eukaryotes, have made them important research tools. The many vectors available, as well as the development of recombinant expression systems, have given yeast an irreplaceable role in the research sector.Y-PER Reagent is effective for S. cerevisiae and other popular species, making it applicable for use in fusion-tagged protein purification and reporter enzyme assays with these model organisms. The lysis reagent also can be used for genomic and plasmid DNA extraction from yeast.

Related Products
Y-PER™ Plus Dialyzable Yeast Protein Extraction Reagent
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form
Liquid
Product Line
Y-PER™
Reagent Type
Detergent Solution, Cell Lysis Buffer
Quantity
200mL
Product Type
Yeast Protein Extraction Reagent

Contents & Storage

Store product at room temperature.

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