Search
Invitrogen
Multispecies p38 MAPK (Phospho) [pT180/pY182] InstantOne™ ELISA Kit
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
![Multispecies p38 MAPK (Phospho) [pT180/pY182] InstantOne™ ELISA Kit](https://www.thermofisher.com/antibody/images/650/p38-phospho-tds.jpg?time=20240507 "Multispecies p38 MAPK (Phospho) [pT180/pY182] InstantOne™ ELISA Kit")
FIGURE: 1 / 3
Multispecies p38 MAPK (Phospho) [pT180/pY182] InstantOne™ ELISA Kit
Representative data for p38 MAPK (Phospho) [pT180/pY182] Multispecies InstantOne™ ELISA. HEK293 cells untreated (-) or treated with 2 µg/mL anisomycin for 30 min (+). Cells were analyzed by immunoblotting (left) and InstantOne™ ELISA (right). The intensity of the band at 38 kDa as detected by immunoblot correlates with InstantOne™ ELISA absorption values.
![Multispecies p38 MAPK (Phospho) [pT180/pY182] InstantOne™ ELISA Kit](https://www.thermofisher.com/antibody/images/150/p38-phospho-tds.jpg?time=20240507)
![Multispecies p38 MAPK (Phospho) [pT180/pY182] InstantOne™ ELISA Kit (85-86022-11)](https://www.thermofisher.com/antibody/images/150/TGR.ELISA.Kit-650x600.jpg?time=20240507)
►
Product Specifications
Sample type/volume
Cell Lysate
20 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
p38 MAPK alpha
Target site-specificity
Phosphorylated Thr180, Phosphorylated Tyr182
Species (tested)
Human, Mouse
Assay kit format
InstantOne ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human and mouse p38 MAPK in cell lysates. Detection of rat p38 MAPK is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
Target information
MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : Crk1, CSBP, CSBP1, CSBP2, CSPB1, EXIP, MAPK14, MXI2, p38, p38-alpha, p38a, p38ALPHA, p38MAPK, PRKM14, PRKM15, RK, SAPK2A
Gene ID : (Human) 1432, (Mouse) 26416
Gene symbol : MAPK14, Mapk14
Protein Aliases : CRK1, CSAID-binding protein, Csaids binding protein, cytokine suppressive anti-inflammatory drug binding protein, cytokine suppressive anti-inflammatory drug binding protein 1, Cytokine suppressive anti-inflammatory drug-binding protein, EC 2.7.11.24, ERK1, ERT2, MAP 14, MAP kinase 1, RP1-179N16.5, MAP kinase p38, MAP kinase p38alpha, MAPK1 antibody, MK14, OTTHUMP00000216563, OTTHUMP00000216566, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14, MAX-interacting protein 2, mitogen activated protein kinase 14, Mitogen-activated protein kinase 14, Mitogen-activated protein kinase p38 alpha, p38 alpha, p38 MAP kinase, p38 MAP kinase alpha, p38 MAPK, p38 mitogen activated protein kinase, p38alpha Exip, SAPK2a, Stress-activated protein kinase 2a, tRNA synthetase cofactor p38
UniProt ID (Human) Q16539, (Mouse) P47811
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support