Search
Invitrogen
Human STAT1 (Phospho) [pY701] InstantOne™ ELISA Kit
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
![Human STAT1 (Phospho) [pY701] InstantOne™ ELISA Kit](https://www.thermofisher.com/antibody/images/650/stat1-phos-tds.jpg?time=20240507 "Human STAT1 (Phospho) [pY701] InstantOne™ ELISA Kit")
FIGURE: 1 / 3
Human STAT1 (Phospho) [pY701] InstantOne™ ELISA Kit
Representative data for STAT1 (Phospho) [pY701] Multispecies InstantOne™ ELISA. A431 cells were either unstimulated (-) or treated with EGF (1 µg/mL for 10 min) (+). Lysates were prepared as described in the InstantOne™ User Manual. Phosphorylated STAT1 (Tyr701) was analyzed in cell lysates by immunoblotting (left) and InstantOne™ ELISA (right) 5 µg/analysis. The intensity of the band as detected by immuno... View More
![Human STAT1 (Phospho) [pY701] InstantOne™ ELISA Kit](https://www.thermofisher.com/antibody/images/150/stat1-phos-tds.jpg?time=20240507)
![Human STAT1 (Phospho) [pY701] InstantOne™ ELISA Kit (85-86092-11)](https://www.thermofisher.com/antibody/images/150/TGR.ELISA.Kit-650x600.jpg?time=20240507)
►
Product Specifications
Sample type/volume
Cell Lysate
20 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
STAT1
Target site-specificity
Assay kit format
InstantOne ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human STAT1 (Tyr701) in cell lysates. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately two hours. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
Target information
STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. The STAT1 gene is located on chromosome 2. STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases : Transcription factor ISGF-3 components p91/p84
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support