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Human SMAD3 (Phospho) [pS423/pS425] InstantOne™ ELISA Kit
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![Human SMAD3 (Phospho) [pS423/pS425] InstantOne™ ELISA Kit](https://www.thermofisher.com/antibody/images/650/pSmad3_tds.jpg?time=20240507 "Human SMAD3 (Phospho) [pS423/pS425] InstantOne™ ELISA Kit")
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Human SMAD3 (Phospho) [pS423/pS425] InstantOne™ ELISA Kit
Representative data for SMAD3 (Phospho) [pS423/pS425] Multispecies InstantOne™ ELISA. C2C12 cells were either unstimulated (-) or treated with TGF beta (50 ng/mL for 90 min) (+). Lysates were prepared as described in the InstantOne™ User Manual. Phosphorylated SMAD3 (Ser423/425) was analyzed in cell lysates by immunoblotting (left) and InstantOne™ ELISA (right) using 10 µg/analysis. The intensity of the b... View More
![Human SMAD3 (Phospho) [pS423/pS425] InstantOne™ ELISA Kit](https://www.thermofisher.com/antibody/images/150/pSmad3_tds.jpg?time=20240507)
![Human SMAD3 (Phospho) [pS423/pS425] InstantOne™ ELISA Kit (85-86192-11)](https://www.thermofisher.com/antibody/images/150/TGR.ELISA.Kit-650x600.jpg?time=20240507)
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Product Specifications
Sample type/volume
Cell Lysate
20 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
SMAD3
Target site-specificity
Phosphorylated Ser423, Phosphorylated Ser425
Species (tested)
Human
Assay kit format
InstantOne ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human SMAD3 (Ser423/425) in cell lysates. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
Suitable Sample Types
Tested on cell culture samples. Expected to work on many other sample types, but have not been tested at this time.
Target information
SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : hMAD-3, hSMAD3, HSPC193, HsT17436, JV15-2, LDS1C, LDS3, mad3, MADH3, SMAD3
Gene ID : (Human) 4088
Gene symbol : SMAD3
Protein Aliases : DKFZP586N0721, JV15-2, MAD homolog 3, mad homolog JV15-2, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MGC60396, SMAD, DKFZp586N0721, DKFZp686J10186, MAD-3, Mothers against decapentaplegic homolog 3, mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3, unnamed protein product
UniProt ID (Human) P84022
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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