PrimeFlow™ Fixation/Permeabilization Buffer Set

The PrimeFlow™ Fixation/Permeabilization Buffer Set is a component of the PrimeFlow™ RNA Assay kit (cat. 88-18001-204 and 88-18009-204). These buffersRead more

Catalog Number	Quantity
88-17000-210	100 Tests

Catalog number 88-17000-210

Price (TWD)

Quantity:

100 Tests

The PrimeFlow™ Fixation/Permeabilization Buffer Set is a component of the PrimeFlow™ RNA Assay kit (cat. 88-18001-204 and 88-18009-204). These buffers can be used to evaluate intracellular staining antibody performance following the fixation and permeabilization steps of the assay. Note that additional changes to antibody performance may be observed after the hybridization steps of the PrimeFlow™ RNA assay.

Please refer to the PrimeFlow™ RNA Assay protocol on the product data sheet for instructions for use.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)Intracellular Staining Followed by Flow Cytometric Analysis

For Use With (Equipment)Flow cytometers

No. of Reactions100 Tests

Product TypeBuffer Set for RNA Assay

Quantity100 Tests

Unit SizeEach

Contents & Storage

2°C to 8°C

Frequently asked questions (FAQs)

When running the QuantiGene FlowRNA (methanol based) assay, which fluorochromes are recommended for antibody staining?

We recommend the use of fluorochromes that are known to be methanol resistant. Avoid protein-based fluorochromes such as APC, PE, and PerCP and their respective tandems. This question is not appropriate for PrimeFlow RNA Assay.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For the PrimeFlow RNA Assay, how would you recommend I set compensation?

We recommend using the PrimeFlow Compensation Kit included in the PrimeFlow RNA Assay (Cat. No. 88-18005-204, 88-18005-210) to set compensation for each RNA channel. Refer to Appendix 3 of the PrimeFlow RNA Assay User Manual for detailed instructions for use. The UltraComp eBeads microspheres included in the compensation kit may also be used for any experimental antibodies being used. Antibodies with the same fluorochromes as the RNA probes should not be used to set compensation for RNA probes, as the compensation values will be different.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have a 37 degrees C tissue culture incubator in the lab. Can I use this incubator for the hybridization steps in the PrimeFlow RNA Assay protocol?

The hybridization temperature is a critical parameter of this protocol necessary to obtain positive results. The incubator to be used must be validated before use with the ViewRNA Temperature Validation Kit (Cat. No. QV0523), following the protocol in the FlowRNA user manual. Temperatures deviated outside of this range will lead to less-than-optimal hybridization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of 96-well plate is compatible with the PrimeFlow RNA protocol?

We recommend polystyrene uncoated v-bottom 96-well plates with a lid (Cat. No. 44-17005-46). The u-bottom plate is also acceptable, but flat bottom plates are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How many cells should I use per well for the 96-well format of the PrimeFlow RNA Assay?

We recommend 1-5 million cells in 100 µL of Flow Cytometry Staining Buffer per well. The starting cell number is critical for maximal signal intensity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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