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Invitrogen
Mouse IL-12/IL-23 p40 (Total) Uncoated ELISA Kit
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Product Specifications
Analytical sensitivity
2 pg/mL
Assay range
2-300 pg/mL
Sample type/volume
Serum
100 µL
Supernatant
100 µL
Hands-on time
1 hr 45 min
Time-to-result
24 hr 30 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
2 x 96 Tests
Contents
Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 µM) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent: 5X concentrated
Detection enzyme: Pre-titrated Avidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 µM) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent: 5X concentrated
Detection enzyme: Pre-titrated Avidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
IL-12/IL-23 p40
Target sub-specificity
Total
Species (tested)
Mouse
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Mouse IL-12/IL-23 p40 (Total) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-12/IL-23 p40 (Total). Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Target information
IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : Il-12a, Il-12b, IL-12p35, Il-12p40, IL-23, Il12p40, Ll12a, p19, p35, p40
Gene ID : (Mouse) 16159, (Mouse) 16160, (Mouse) 83430
Gene symbol : Il12a, Il12b, Il23a
Protein Aliases : CLMF p35, CLMF p40, cytotoxic lymphocyte maturation factor 35 kDa subunit, cytotoxic lymphocyte maturation factor 40 kDa subunit, il 12, IL 12/IL 23p40, il 23, IL 23 p40, IL12 p40, IL23, il23, IL23 p41, ILN, Interleukin, Interleukin 12, Interleukin12, RP23-388G23.1, IL-12 p35 subunit, IL-12 p40 subunit, IL-12 subunit p35, IL-12 subunit p40, IL-23 subunit alpha, IL-23 subunit p40, IL-23-A, IL-23p19, interleukin 12a p35 subunit, interleukin-12 p35 subunit, interleukin-12 p40 subunit, interleukin-23 subunit p19, unnamed protein product
UniProt ID (Mouse) P43431, (Mouse) P43432, (Mouse) Q9EQ14
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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