HisPur Ni-NTA resin performs as well or better than other suppliers' nickel resins
Gel panels: Bacterial lysate containing over-expressed 6xHis-AIF2 (6mg total protein) or 6xHis-GFP (4mg total protein) was applied to HisPur Ni-NTA Resin (0.2mL) and purified by the batch-bind method. The same amount of total protein was applied to Ni-IDA and HisPur Cobalt Resins and purified as described in the instructions. Gels lanes were normalized to equivalent volume. M = molecular-weight marker, L = lysate load and FT = flow-through.
HisPur Ni-NTA Resin can be used at least five times without losing performance
HisPur Ni-NTA and HisPur Cobalt Resins maximize yield and purity
HisPur Ni-NTA resin is effective for purifying His-tagged proteins in batch-bind and spin-column formats
|Column Type:||Affinity,Agarose Resin|
|Immobilized Phase:||Divalent Nickel,Metal Chelate|
|Product Size:||25 columns|