Thermo Scientific™

RPMI 1640 Medium for SILAC

Catalog number: 88365
Thermo Scientific™

RPMI 1640 Medium for SILAC

Catalog number: 88365
Catalog Number
88365
Unit Size
500 mL
Price (USD)
64.25
Your Price:
Quantity
Catalog NumberUnit SizePrice (USD)Quantity
88365500 mL
64.25
Your Price:
Product Overview
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Thermo Scientific™ RPMI 1640 Medium for SILAC is optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).

Features of RPMI 1640 Medium for SILAC:
• Flexible—liquid media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling
• High quality—medium is sterile, endotoxin-free, and cell culture-compatible

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of 'heavy' 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization, and quantitation of proteins.

This SILAC medium is used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis. SILAC media supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells.

Related products
NeuCode™ Lysine -080, 50 mg
Fetal Bovine Serum, dialyzed, US origin
DMEM for SILAC

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Shipping Condition
Room Temperature
Detection Method
Mass Spectrometry
Final Product Type
Liquid Medium
For Use With (Equipment)
Mass Spectrometer
Label Type
Mass Isotopes of Amino Acids
Starting Material
Protein samples
Workflow Step
Cell Culture
Material
Protein samples
Product Type
Media
Quantity
500mL

Contents & Storage

1 x 500 mL, store at 2–8°C

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