1-Step Human Coupled IVT Kit - DNA
1-Step Human Coupled IVT Kit - DNA
Thermo Scientific™

1-Step Human Coupled IVT Kit - DNA

Thermo Scientific 1-Step Human In Vitro Protein Expression Kits enable the translation and post-transcriptional modification of full-length proteins from mRNARead more
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Catalog NumberQuantity
8888240 Reaction Kit
888818 Reaction Kit
Catalog number 88882
Price (USD)/ 40 reactions
872.65
Online exclusive
918.00 
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Quantity:
40 Reaction Kit
Request bulk or custom format
Price (USD)/ 40 reactions
872.65
Online exclusive
918.00 
Save 45.35 (5%)
Add to cart
1-Step Human Coupled IVT Kit - DNA
Catalog number88882
Price (USD)/ 40 reactions
872.65
Online exclusive
918.00 
Save 45.35 (5%)
-
Add to cart
Thermo Scientific 1-Step Human In Vitro Protein Expression Kits enable the translation and post-transcriptional modification of full-length proteins from mRNA or plasmid templates with yields of up to 100 μg/mL per reaction.

The Human IVT Kits are unique HeLa cell lysate-based protein expression systems for in vitro translation or coupled transcription/translation reactions. Protein expression is performed in a single 90-minute reaction that can be extended for up to 6 hours with continued protein production up to 100 μg/mL when combined with the optimized pT7CFE1 Expression Vector. The Human IVT Kits can express functional enzymes, phosphoproteins, glycoproteins and membrane proteins for immediate use in studying protein interactions, performing rapid mutational analysis and measuring activity.

Features of the 1-Step Human Coupled IVT Kit:

Functional—uses the human translational machinery to express active proteins
Convenient—perform transcription and translation in a single step
High performance—greater yields compared to rabbit reticulocyte in vitro translation
Reliable—express proteins that fail in rabbit reticulocyte systems

Better Than Traditional Methods:
• HeLa cell-free extracts are capable of expressing proteins with post-translational modifications
• Accurate translation delivers full-length protein compatible with downstream applications
• Protein translation is optimized with EMCV IRES element and other mRNA stabilizing elements

Includes:
• Complete kits contain HeLa cell lysate, accessory proteins, reaction mix, nuclease-free water, expression vector and a GFP-positive control vector

Requires:
• 30°C incubator or water bath

Applications:
• Express proteins to measure enzyme activity
• Perform mutational analysis
• Express protein for use in gel mobility shift assays
• Perform co-immunoprecipitation
• Express cytotoxic proteins
• Unnatural amino acid labeling
• Identification and validation of transcriptome small molecule inhibitors
• Screening of RNA targeted small molecules to modify translation

The 1-Step Coupled Human IVT Kit for DNA is a cell-free system using the cellular transcription and translation machinery from a modified HeLa cell line. The procedure is quick and easy and is an effective alternative to other protein expression methods. Simply add an appropriate expression construct to a mixture of HeLa cell lysate, Accessory Proteins, Reaction Mix and incubate at 30°C for 90 minutes to overnight.

The 1-Step Human IVT Kit for mRNA also uses the human protein translation machinery to produce functional protein. This kit is recommended for translation of mRNA transcripts containing an EMCV IRES element and mRNA stabilizing features designed into the pT7CFE1 expression vector.

The human in vitro translation system is robust and will express proteins from a variety of species including mammals, bacteria and protozoa. Benchmarking shows that the expression levels of functional proteins such as luciferase are much higher in the human system compared to either rabbit reticulocyte-based or E. coli-based systems. Furthermore, proteins expressed with the human in vitro translation system are not contaminated with substances that can interfere with downstream applications.

More Product Data
Protocol for in vitro protein expression using mRNA templates
Protein Biology Application Notes
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Type1-step Human Coupled IVT Kit
Sufficient For40 Reactions of 25 μL Each
Quantity40 Reaction Kit
Expression SystemCell-Free
Expressed Protein TypeMembrane Protein
Unit Size40 reactions
Contents & Storage
Sufficient For: 40 reactions of 25 μL each
• HeLa Lysate, 500 μL
• Accessory Proteins, 100 μL
• Reaction Mix, 200 μL
• Positive Control DNA: pCFE-GFP, 10 μg
• pT7CFE1-CHis, 10 μg
• Nuclease-Free Water, 1.5 mL

Upon receipt store kit at -80°C. Product is shipped on dry ice