Thermo Scientific Pierce Human Rhinovirus (HRV) 3C Protease is a recombinant cysteine protease used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.
Features of Thermo Scientific Pierce HRV 3C Protease:
• Effective—HRV 3C is designed to remove purification tags from your protein of interest • High activity at 4°C—activity is ≥2000U/mg control protein; one unit will cleave 0.1 mg control protein at 4°C after 16h • Flexible—dual-tagged enzyme is compatible with various columns and buffers
Properties: • Alternative names: Human Rhinovirus 3C • Source: Escherichia coli (E.coli) • Molecular weight: 47.8kD • Form: Liquid • Protease type: Cysteine • Optimal reaction conditions: 50 mM Tris-HCl, 150 mM NaCl, pH 8.0
Pierce HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro and cleaves after the glutamine residue. The recombinant enzyme is engineered as a fusion protein with glutathione-S-transferase (GST) and polyhistidine (6xHis) tags, so that the protease can be easily removed after cleavage using a variety of solid supports with immobilized glutathione (GSH) or metal chelates, respectively. HRV3C is also active in a variety of commonly used buffers, providing further flexibility in experimental design.
Applications: • Removal of purification tags from fusion proteins