Pierce™ Glycoprotein Isolation Kit, ConA

Catalog number: 89804

Thermo Scientific™  Related applications: Protein Purification & Isolation

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Description

The Thermo Scientific Pierce Glycoprotein Isolation Kit (Concanavilin A) allows isolation of glycoproteins from complex mixtures, including serum, tissue and cultured cell lysates. The procedure uses concanavilin A (ConA) as a ligand to bind a specific class of glycoprotein from complex protein samples—those containing alpha-lined mannose or terminal glucose residues. This kit has been used to isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.

Features of this Glycoprotein Isolation Kit:

High recovery—equivalent or greater glycoprotein recovery versus competitor kits and lectin resins
Fast—glycoprotein purification in less than an hour
Versatile—isolate glycoproteins from various sample types, e.g., human serum and cell lysate.
Robust—lectin does not leach from resin when processing sample
Convenient—complete kit offering lectin resins and spin columns with all necessary reagents
Compatible with Bradford-based protein assays—dialysis or protein precipitation of recovered glycoproteins is not required prior to protein assay

Related Products
Pierce™ Glycoprotein Isolation Kit, WGA
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Spin Column, Kit, Microcentrifuge
Column Type: Affinity, Agarose Resin
Product Size: 10 reactions
Immobilized Phase: Lectin

Contents & storage

Sufficient For: Isolating glycoproteins with strong affinity for ConA from 10 samples of up to 640 µL (1 to 1.5 mg total protein) each
• ConA Lectin Resin, 1.1 mL
• Binding/Wash Buffer (5X), 6.5 mL
• Elution Buffer, 5 mL
• Spin Columns, 10 columns
• Collection Tubes, 20 tubes

Store at 4°C.

Documents

Manuals & protocols