Thermo Scientific™

Pierce™ Glycoprotein Isolation Kit, WGA

Catalog number: 89805
Thermo Scientific™

Pierce™ Glycoprotein Isolation Kit, WGA

Catalog number: 89805
Catalog Number
89805
Unit Size
10 reactions
Price (USD)
Price: 467.00
Online price: 452.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
8980510 reactions
Price: 467.00
Online price: 452.65
Your price:
-
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The Thermo Scientific Pierce Glycoprotein Isolation Kit (Wheat Germ Agglutinin) allows isolation of glycoproteins from complex mixtures, including serum, tissue and cultured cell lysates. The procedure uses wheat germ agglutinin (WGA) as a ligand to bind a specific class of glycoprotein from complex protein samples—those containing N-Acetyl glucosamine (GlcNAc) groups or sialic acid. This kit has been used to isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.

Features of this Glycoprotein Isolation Kit:

High recovery—equivalent or greater glycoprotein recovery versus competitor kits and lectin resins
Fast—glycoprotein purification in less than an hour
Versatile—isolate glycoproteins from various sample types, e.g., human serum and cell lysate.
Robust—lectin does not leach from resin when processing sample
Convenient—complete kit offering lectin resins and spin columns with all necessary reagents
Compatible with Bradford-based protein assays—dialysis or protein precipitation of recovered glycoproteins is not required prior to protein assay

Related Products
Pierce™ Glycoprotein Isolation Kit, ConA
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Description
Pierce Glycoprotein Isolation Kit, WGA
Product Line
Pierce™
Quantity
1 Kit
Target
Glycoprotein
Type
Glycoprotein Isolation Kit
Stationary Phase
Lectin
Sufficient For
10 Reactions
Format
Kit, Microcentrifuge, Spin Column
Column Type
Affinity, Agarose Resin

Contents & Storage

Sufficient For: Isolating glycoproteins with strong affinity for WGA from 10 samples of up to 640 μL (1 to 1.5 mg total protein) each
• WGA Lectin Resin, 1.1 mL
• Binding/Wash Buffer (5X), 6.5 mL
• Elution Buffer, 5 mL
• Spin Columns, 10 columns
• Collection Tubes, 20 tubes

Store at 4°C.

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