P-Rex1 Mediates Glucose-Stimulated Rac1 Activation and Insulin Secretion in Pancreatic β-Cells.
AuthorsThamilselvan V,Gamage S,Harajli A,Chundru SA,Kowluru A
JournalCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
PubMed ID33347743
BACKGROUND/AIMS: Despite the published evidence implicating phosphoinositide 3-kinase (PI3-kinase) in the regulation of islet function, limited information is available on the putative contributory roles of its downstream signaling steps, including the phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1 (P-Rex1) signaling pathway in the islet β-cell. Therefore, we investigated potential roles for P-Rex1 ... More
Baicalein promotes KDM4E to induce BICD1 and inhibit triple-negative breast cancer progression by blocking PAR1 signaling.
Baicalein has been implicated in the chemotherapy overcoming triple-negative breast cancer (TNBC). However, many unanswered questions remain regarding its role in treating TNBC. Here, we sought to demonstrate the molecular pathway mediated by baicalein in TNBC. Lysine-specific demethylase 4E (KDM4E), reduced in TNBC cells, was identified as a target protein ... More
Exposure to Hypoxic Conditions Up-regulates HER2 in Breast Cancer Cell Lines.
AuthorsSuganuma N,Saito N,Yasukawa M,Yamanaka T,Yamashita T,Miyagi Y,Saito A,Hoshino D
JournalAnticancer research
PubMed ID39626936
BACKGROUND/AIM: Tissue specimen quality is becoming increasingly important for basic research and routine clinical results. Warm ischemia time (WIT) affects human epidermal growth factor receptor 2 (HER2) immunohistochemistry (IHC) scores. However, the role of WIT on HER2 modulation remains unclear. We hypothesized that the WIT-mediated increase in HER2 IHC scores ... More
Selective Enrichment of Membrane Proteins by Partition Phase Separation for Proteomic Studies.
AuthorsQoronfleh MW, Benton B, Ignacio R, Kaboord B
JournalJ Biomed Biotechnol
PubMed ID14615633
'The human proteome project will demand faster, easier, and more reliable methods to isolate and purify protein targets. Membrane proteins are the most valuable group of proteins since they are the target for 70-80% of all drugs. Perbio Science has developed a protocol for the quick, easy, and reproducible isolation ... More
Ubl4A is required for insulin-induced Akt plasma membrane translocation through promotion of Arp2/3-dependent actin branching.
AuthorsZhao Y, Lin Y, Zhang H, Mañas A, Tang W, Zhang Y, Wu D, Lin A, Xiang J
Journal
PubMed ID26195787
'The serine-threonine kinase Akt is a key regulator of cell proliferation and survival, glucose metabolism, cell mobility, and tumorigenesis. Activation of Akt by extracellular stimuli such as insulin centers on the interaction of Akt with PIP3 on the plasma membrane, where it is subsequently phosphorylated and activated by upstream protein ... More
Glucagon-like peptide 1 inhibits cell apoptosis and improves glucose responsiveness of freshly isolated human islets.
AuthorsFarilla L, Bulotta A, Hirshberg B, Li Calzi S, Khoury N, Noushmehr H, Bertolotto C, Di Mario U, Harlan DM, Perfetti R
JournalEndocrinology
PubMed ID12960095
The peptide hormone, glucagon-like peptide 1 (GLP-1), has been shown to increase glucose-dependent insulin secretion, enhance insulin gene transcription, expand islet cell mass, and inhibit beta-cell apoptosis in animal models of diabetes. The aim of the present study was to evaluate whether GLP-1 could improve function and inhibit apoptosis in ... More
EGFR inhibition evokes innate drug resistance in lung cancer cells by preventing Akt activity and thus inactivating Ets-1 function.
AuthorsPhuchareon J, McCormick F, Eisele DW, Tetsu O
Journal
PubMed ID26150526
Nonsmall cell lung cancer (NSCLC) is the leading cause of cancer death worldwide. About 14% of NSCLCs harbor mutations in epidermal growth factor receptor (EGFR). Despite remarkable progress in treatment with tyrosine kinase inhibitors (TKIs), only 5% of patients achieve tumor reduction >90%. The limited primary responses are attributed partly ... More