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RIPA Lysis and Extraction Buffer
인용 및 참조 문헌 (7)
Thermo Scientific™

RIPA Lysis and Extraction Buffer

Thermo Scientific RIPA Lysis and Extraction Buffer는 즉시 사용 가능하고 완전히 공개된 고품질 제형의 인기 있는 세포 용해 시약으로 배양된자세히 알아보기
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카탈로그 번호수량
89900100 mL
89901250 mL
카탈로그 번호 89900
제품 가격(KRW)
159,000
온라인 행사
Ends: 31-Mar-2026
176,000
할인액 17,000 (10%)
Each
수량:
100 mL
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
159,000
온라인 행사
Ends: 31-Mar-2026
176,000
할인액 17,000 (10%)
Each
Thermo Scientific RIPA Lysis and Extraction Buffer는 즉시 사용 가능하고 완전히 공개된 고품질 제형의 인기 있는 세포 용해 시약으로 배양된 포유류 세포에서 적합합니다.

RIPA Buffer의 특징:

• 편의성—즉시 사용 가능한 용액. 직접 구성품을 조립하고 준비할 필요가 없음
• 융통성—리포터 분석, 단백질 분석, 면역분석, 단백질 정제를 포함한 다양한 어플리케이션에서 사용 가능
• 활용성—세포질 단백질, 막(membrane) 단백질, 핵 단백질 추출 가능
• 공개된 제형—고유한 성분을 함유하지 않아 사용자가 가능한 호환성 문제를 완벽하게 통제하고 이해할 수 있음

이 RIPA 버퍼는 플레이트에 든 세포와 팻릿화된 현탁 세포를 포함한 배양된 포유류 세포에서 단백질을 효과적으로 용해하고 추출합니다. 인기 있는 이 시약을 사용해 막(membrane) 단백질, 핵 단백질, 세포질 단백질을 추출하고, 리포터 분석, Thermo Scientific BCA Protein Assay, 면역분석, 단백질 정제를 포함한 다양한 어플리케이션에서 사용할 수 있습니다. Thermo Scientific Halt Protease Inhibitor Cocktail(Part No. 78430) 및 Halt Phosphatase Inhibitor Cocktail(Part No. 78420)와 같은 억제제도 이 RIPA 버퍼 제형에서 사용할 수 있고, 사용 전에 첨가하여 단백질 가수분해를 방지하고 단백질 인산화를 유지할 수 있습니다.

RIPA 버퍼 이름은 최초로 개발된 어플리게이션 이름인 방사선 면역침강(radio-immunoprecipitation) 분석에서 유래되었습니다. 이 동위원소 분석법은 현재 실험실에서 잘 실시되지 않지만, 이 용해 버퍼 제형의 머리글자는 계속 많이 사용되고 있습니다. RIPA 세포 용해 시약은 이온 세척제와 비이온 세척제 3개를 함유하기 때문에 다양한 세포 유형에서 단백질을 추출하는 데 매우 효과적입니다. 이 시약 제형의 한 가지 단점은 다른 용해 시약에 비해 특정 후속 어플리케이션에서 다소 사용할 수 없다는 점입니다.

관련 제품:
Pierce IP Lysis Buffer
M-PER Mammalian Protein Extraction Reagent
Pierce Co-Immunoprecipitation Kit
For Research Use Only. Not for use in diagnostic procedures.

RIPA buffer derives its name from the original application for which it was developed: the radioimmunoprecipitation assay. While this isotopic assay method is rarely performed in laboratories today, the acronym for this lysis buffer formulation has endured in common use. RIPA cell lysis reagent is highly effective for protein extraction from a variety of cell types because it contains three non-ionic and ionic detergents. One disadvantage of this detergent formulation is its relative incompatibility with certain downstream applications compared to other lysis reagents.

General References:

  1. Berman, H.A., et al. (1985). Biochemistry 24, 7140-7147.
  2. Cai, X., et al. (1994). J. Virol. 68(11), 7609-7613.
  3. Cao, F., et al. (2005). J Virol. 79(16), 10164-70.
  4. Cerione, R.A., et al. (1984). Biochemistry 23, 4519-4525.
  5. Elzinga, M., et al. (1984). Proc. Natl. Acad. Sci. (USA) 81, 6599-6602.
  6. Necessary, P.C., et al. (1984). J. Biol. Chem. 259, 6942-6946.
  7. Pfrepper, K.I. and Flugel R.M. (2005). Methods in Mol. Biol. 304, 435-44.
  8. Rouse, J.C. and Vath, J.E. (1996). Anal. Biochem. 238, 83-92
  9. Sefton, B.M. (2005). Unit 18.2. in Ausubel, F.M., et al. (eds.) Current Protocols in Molecular Biology. John Wiley and Sons, Inc.
  10. Sibley, D.R., et al. (1986). Proc. Natl. Acad. Sci. (USA) 83, 9408-9412.
  11. Zhang, J., et al. (1995). J. Biol. Chem. 270(12), 6589-6594.
사양
형식Liquid
수량100 mL
부피(미터법)100 mL
제품 유형Extraction Buffer
Unit SizeEach
구성 및 보관
Upon receipt store at 4°C.

자주 묻는 질문(FAQ)

What are the standard lysis buffers used with mammalian cells for detection of protein expression by immunoprecipitation (IP) or Western blot analysis?

The most commonly used buffer is RIPA Buffer with SDS. We offer RIPA Buffer (Cat. Nos. 89900 and 89901). We also offer the Pierce IP Lysis buffer (Cat. Nos. 87787 and 87788) as well as M-PER (Cat. Nos. 78501, 78503, and 78505).

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

Does RIPA Lysis and Extraction Buffer (Cat. No. 89900, 89901) contain protease or phosphatase inhibitors?

RIPA Lysis and Extraction Buffer (Cat. No. 89900, 89901) does not contain protease or phosphatase inhibitors. If desired, you may add protease and/or phosphatase inhibitors, such as Halt Protease Inhibitor Cocktail (Cat. No. 78410) and Halt Phosphatase Inhibitor Cocktail (Cat. No. 78420) to the RIPA Lysis and Extraction Buffer to prevent proteolysis and maintain phosphorylation status of proteins. We recommend adding protease and phosphatase inhibitors immediately before use.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Why is it not recommended that I use RIPA buffer for protein A280 measurements with my NanoDrop spectrophotometer?

RIPA buffer produces a particularly strong absorbance signal at the 280 nm wavelength. As a result, it will either over estimate or under estimate protein concentrations and interfere with the protein purity ratio.
Protein samples in RIPA buffer should be quantified via the Pierce Protein 660 or BCA colorimetric assays using a full spectrum NanoDrop model.

Find additional tips, troubleshooting help, and resources within ourProtein Purification and Isolation Support Center.

Why is there low phosphorylation of the proteins when I use the RIPA Lysis and Extraction Buffer?

Low phosphorylation is usually due to phosphatase activity. We recommend adding a Halt Phosphatase Inhibitor Cocktail to the buffer before use.
Alternatively, the protein is not phosphorylated or phosphorylated at a low level.

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

What if proteolysis occurs when I use RIPA Lysis and Extraction Buffer?

Proteolysis indicates that no protease inhibitors were added. We recommend adding a Halt Protease Inhibitor Cocktail to the RIPA Lysis and Extraction Buffer before use.

Find additional tips, troubleshooting help, and resources within ourProtein Purification and Isolation Support Center.

View all RIPA Lysis and Extraction Buffer FAQs

인용 및 참조 문헌 (7)

인용 및 참조 문헌
Abstract
Shatavari supplementation in postmenopausal women alters the skeletal muscle proteome and pathways involved in training adaptation.
Authors:O'Leary MF,Jackman SR,Bowtell JL
Journal:European journal of nutrition
PubMed ID:38214710
PURPOSE: Shatavari is an understudied, widely available herbal supplement. It contains steroidal saponins and phytoestrogens. We previously showed that six weeks of shatavari supplementation improved handgrip strength and increased markers of myosin contractile function. Mechanistic insights into shatavari's actions are limited. Therefore, we performed proteomics on vastus lateralis (VL) samples ... More
Cerebral microvascular endothelial cell-derived extracellular vesicles regulate blood - brain barrier function.
Authors:Hosseinkhani B,Duran G,Hoeks C,Hermans D,Schepers M,Baeten P,Poelmans J,Coenen B,Bekar K,Pintelon I,Timmermans JP,Vanmierlo T,Michiels L,Hellings N,Broux B
Journal:Fluids and barriers of the CNS
PubMed ID:38114994
Autoreactive T lymphocytes crossing the blood-brain barrier (BBB) into the central nervous system (CNS) play a crucial role in the initiation of demyelination and neurodegeneration in multiple sclerosis (MS). Recently, extracellular vesicles (EV) secreted by BBB endothelial cells (BBB-EC) have emerged as a unique form of cell-to-cell communication that contributes ... More
Optimization of a Protocol for Protein Extraction from Calcified Aortic Valves for Proteomics Applications: Development of a Standard Operating Procedure.
Authors:Trindade F,Ferreira AF,Saraiva F,Martins D,Mendes VM,Sousa C,Gavina C,Leite-Moreira A,Manadas B,Falcão-Pires I,Vitorino R
Journal:Proteomes
PubMed ID:36136308
The comprehension of the pathophysiological mechanisms, the identification of druggable targets, and putative biomarkers for aortic valve stenosis can be pursued through holistic approaches such as proteomics. However, tissue homogenization and protein extraction are made difficult by tissue calcification. The reproducibility of proteome studies is key in clinical translation of ... More
TGFB1-induced extracellular expression of TGFBIp and inhibition of TGFBIp expression by RNA interference in a human corneal epithelial cell line.
Authors:Yellore VS, Rayner SA, Aldave AJ
Journal:Invest Ophthalmol Vis Sci
PubMed ID:20881301
'To report the increased production of extracellular transforming growth factor ß-induced protein (TGFBIp) by human corneal epithelial cells (HCECs) after induction by TGFB1 and the inhibition of TGFBIp production in induced and noninduced HCECs by RNA interference (RNAi).' ... More
Repair of full-thickness femoral condyle cartilage defects using allogeneic synovial cell-engineered tissue constructs.
Authors:Pei M, He F, Boyce BM, Kish VL
Journal:Osteoarthritis Cartilage
PubMed ID:19128988
Synovium-derived stem cells (SDSCs) have proven to be superior in cartilage regeneration compared with other sources of mesenchymal stem cells. We hypothesized that conventionally passaged SDSCs can be engineered in vitro into cartilage tissue constructs and the engineered premature tissue can be implanted to repair allogeneic full-thickness femoral condyle cartilage ... More
View all RIPA Lysis and Extraction Buffer citations