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RIPA Lysis and Extraction Buffer
引用資料與參考文獻 (7)
Thermo Scientific™

RIPA Lysis and Extraction Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.
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產品號碼Quantity
89900100 mL
89901250 mL
產品號碼 89900
價格 (HKD)
869.00
線上優惠
Ends: 30-Jun-2026
1,103.00
您節省 234.00 (21%)
Each
Quantity:
100 mL
Request bulk or custom format
價格 (HKD)
869.00
線上優惠
Ends: 30-Jun-2026
1,103.00
您節省 234.00 (21%)
Each

Thermo Scientific RIPA Lysis and Extraction Buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.

Features of RIPA Buffer:

  • Convenient—ready-to-use solution; no need to assemble and prepared components yourself
  • Flexible—compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification
  • Versatile—enables extraction of cytoplasmic, membrane and nuclear proteins
  • Disclosed formulation—contains no proprietary components, providing users with complete control and knowledge of possible compatibility issues

This RIPA buffer effectively lyses and extracts protein from cultured mammalian cells, including plated cells and pelleted suspension cells. The popular reagent enables the extraction of membrane, nuclear and cytoplasmic proteins and is compatible with many applications, including reporter assays, the Thermo Scientific BCA Protein Assay (Cat. No. 23225), immunoassays and protein purification. Inhibitors such as Thermo Scientific Halt Protease Inhibitor Cocktail (Cat. No. 78420) and Halt Phosphatase Inhibitor Cocktail (Cat. No. 78420) are also compatible with this RIPA buffer formulation and can be added before use to prevent proteolysis and maintain protein phosphorylation.

RIPA buffer derives its name from the original application for which it was developed: the radio-immunoprecipitation assay. While this isotopic assay method is rarely performed in laboratories today, the acronym for this lysis buffer formulation has endured in common use. RIPA cell lysis reagent is highly effective for protein extraction from a variety of cell types because it contains three non-ionic and ionic detergents. One disadvantage of this detergent formulation is its relative incompatibility with certain downstream applications compared to other lysis reagents.

Related Products

  • Pierce™ IP Lysis Buffer (Cat. No. 87787)
  • M-PER™ Mammalian Protein Extraction Reagent (Cat. No. 78501)
For Research Use Only. Not for use in diagnostic procedures.

RIPA buffer derives its name from the original application for which it was developed: the radioimmunoprecipitation assay. While this isotopic assay method is rarely performed in laboratories today, the acronym for this lysis buffer formulation has endured in common use. RIPA cell lysis reagent is highly effective for protein extraction from a variety of cell types because it contains three non-ionic and ionic detergents. One disadvantage of this detergent formulation is its relative incompatibility with certain downstream applications compared to other lysis reagents.

General References:

  1. Berman, H.A., et al. (1985). Biochemistry 24, 7140-7147.
  2. Cai, X., et al. (1994). J. Virol. 68(11), 7609-7613.
  3. Cao, F., et al. (2005). J Virol. 79(16), 10164-70.
  4. Cerione, R.A., et al. (1984). Biochemistry 23, 4519-4525.
  5. Elzinga, M., et al. (1984). Proc. Natl. Acad. Sci. (USA) 81, 6599-6602.
  6. Necessary, P.C., et al. (1984). J. Biol. Chem. 259, 6942-6946.
  7. Pfrepper, K.I. and Flugel R.M. (2005). Methods in Mol. Biol. 304, 435-44.
  8. Rouse, J.C. and Vath, J.E. (1996). Anal. Biochem. 238, 83-92
  9. Sefton, B.M. (2005). Unit 18.2. in Ausubel, F.M., et al. (eds.) Current Protocols in Molecular Biology. John Wiley and Sons, Inc.
  10. Sibley, D.R., et al. (1986). Proc. Natl. Acad. Sci. (USA) 83, 9408-9412.
  11. Zhang, J., et al. (1995). J. Biol. Chem. 270(12), 6589-6594.
規格
FormatLiquid
Quantity100 mL
Volume (Metric)100 mL
Product TypeExtraction Buffer
Unit SizeEach
內容物與存放
Upon receipt store at 4°C.

常見問答集 (常見問題)

What are the standard lysis buffers used with mammalian cells for detection of protein expression by immunoprecipitation (IP) or Western blot analysis?

The most commonly used buffer is RIPA Buffer with SDS. We offer RIPA Buffer (Cat. Nos. 89900 and 89901). We also offer the Pierce IP Lysis buffer (Cat. Nos. 87787 and 87788) as well as M-PER (Cat. Nos. 78501, 78503, and 78505).

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

Does RIPA Lysis and Extraction Buffer (Cat. No. 89900, 89901) contain protease or phosphatase inhibitors?

RIPA Lysis and Extraction Buffer (Cat. No. 89900, 89901) does not contain protease or phosphatase inhibitors. If desired, you may add protease and/or phosphatase inhibitors, such as Halt Protease Inhibitor Cocktail (Cat. No. 78410) and Halt Phosphatase Inhibitor Cocktail (Cat. No. 78420) to the RIPA Lysis and Extraction Buffer to prevent proteolysis and maintain phosphorylation status of proteins. We recommend adding protease and phosphatase inhibitors immediately before use.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Why is it not recommended that I use RIPA buffer for protein A280 measurements with my NanoDrop spectrophotometer?

RIPA buffer produces a particularly strong absorbance signal at the 280 nm wavelength. As a result, it will either over estimate or under estimate protein concentrations and interfere with the protein purity ratio.
Protein samples in RIPA buffer should be quantified via the Pierce Protein 660 or BCA colorimetric assays using a full spectrum NanoDrop model.

Find additional tips, troubleshooting help, and resources within ourProtein Purification and Isolation Support Center.

Why is there low phosphorylation of the proteins when I use the RIPA Lysis and Extraction Buffer?

Low phosphorylation is usually due to phosphatase activity. We recommend adding a Halt Phosphatase Inhibitor Cocktail to the buffer before use.
Alternatively, the protein is not phosphorylated or phosphorylated at a low level.

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

What if proteolysis occurs when I use RIPA Lysis and Extraction Buffer?

Proteolysis indicates that no protease inhibitors were added. We recommend adding a Halt Protease Inhibitor Cocktail to the RIPA Lysis and Extraction Buffer before use.

Find additional tips, troubleshooting help, and resources within ourProtein Purification and Isolation Support Center.

View all RIPA Lysis and Extraction Buffer FAQs

引用資料與參考文獻 (7)

引用資料與參考文獻
Abstract
Shatavari supplementation in postmenopausal women alters the skeletal muscle proteome and pathways involved in training adaptation.
Authors:O'Leary MF,Jackman SR,Bowtell JL
Journal:European journal of nutrition
PubMed ID:38214710
PURPOSE: Shatavari is an understudied, widely available herbal supplement. It contains steroidal saponins and phytoestrogens. We previously showed that six weeks of shatavari supplementation improved handgrip strength and increased markers of myosin contractile function. Mechanistic insights into shatavari's actions are limited. Therefore, we performed proteomics on vastus lateralis (VL) samples ... More
Cerebral microvascular endothelial cell-derived extracellular vesicles regulate blood - brain barrier function.
Authors:Hosseinkhani B,Duran G,Hoeks C,Hermans D,Schepers M,Baeten P,Poelmans J,Coenen B,Bekar K,Pintelon I,Timmermans JP,Vanmierlo T,Michiels L,Hellings N,Broux B
Journal:Fluids and barriers of the CNS
PubMed ID:38114994
Autoreactive T lymphocytes crossing the blood-brain barrier (BBB) into the central nervous system (CNS) play a crucial role in the initiation of demyelination and neurodegeneration in multiple sclerosis (MS). Recently, extracellular vesicles (EV) secreted by BBB endothelial cells (BBB-EC) have emerged as a unique form of cell-to-cell communication that contributes ... More
Optimization of a Protocol for Protein Extraction from Calcified Aortic Valves for Proteomics Applications: Development of a Standard Operating Procedure.
Authors:Trindade F,Ferreira AF,Saraiva F,Martins D,Mendes VM,Sousa C,Gavina C,Leite-Moreira A,Manadas B,Falcão-Pires I,Vitorino R
Journal:Proteomes
PubMed ID:36136308
The comprehension of the pathophysiological mechanisms, the identification of druggable targets, and putative biomarkers for aortic valve stenosis can be pursued through holistic approaches such as proteomics. However, tissue homogenization and protein extraction are made difficult by tissue calcification. The reproducibility of proteome studies is key in clinical translation of ... More
TGFB1-induced extracellular expression of TGFBIp and inhibition of TGFBIp expression by RNA interference in a human corneal epithelial cell line.
Authors:Yellore VS, Rayner SA, Aldave AJ
Journal:Invest Ophthalmol Vis Sci
PubMed ID:20881301
'To report the increased production of extracellular transforming growth factor ß-induced protein (TGFBIp) by human corneal epithelial cells (HCECs) after induction by TGFB1 and the inhibition of TGFBIp production in induced and noninduced HCECs by RNA interference (RNAi).' ... More
Repair of full-thickness femoral condyle cartilage defects using allogeneic synovial cell-engineered tissue constructs.
Authors:Pei M, He F, Boyce BM, Kish VL
Journal:Osteoarthritis Cartilage
PubMed ID:19128988
Synovium-derived stem cells (SDSCs) have proven to be superior in cartilage regeneration compared with other sources of mesenchymal stem cells. We hypothesized that conventionally passaged SDSCs can be engineered in vitro into cartilage tissue constructs and the engineered premature tissue can be implanted to repair allogeneic full-thickness femoral condyle cartilage ... More
View all RIPA Lysis and Extraction Buffer citations